Pressure myograph system for rapid freezing - 115FP

Pressure myograph system for rapid freezing  - 115FP DMT

Pressure myograph system for rapid freezing - 115FP

The Pressure Myograph System - 115FP is designed for bio-chemical or morphological studies where rapid freezing or fixing of the intact, pressurized vessel is required. After rap-id freezing or fixation, the vessel can be used in studies such as morphological analysis or immunohistochemistry.

Přidat žádost o nabídku
  • Popis

Pressure myograph system for rapid freezing - 115FP

FEATURES & BENEFITS

  • Ideal for rapid freezing or fixation of vessel segments for biochemical or morphological assays after the functional experiment
  • Flexible for studying the structure and function of small vessels from >60 μm with the ability to rapidly freeze/fix the vessel in the pressurized state
  • Features a special chamber made of inert POM to withstand low temperatures and fixatives
  • Built-in heating, ideal for pharmacological reactivity work

The Pressure Myograph System - 115FP is designed for bio-chemical or morphological studies where rapid freezing or fixing of the intact, pressurized vessel is required. After rap-id freezing or fixation, the vessel can be used in studies such as morphological analysis or immunohistochemistry.
A built-in heating system maintains the chamber tempera-ture, eliminating the need for continuous (and often costly) superfusion. The chamber cover includes ports for superfu-sion, for rapid draining and filling, for cumulative addition of drugs and for oxygenation. To facilitate cleaning, the cham-ber is made of acid resistant  stainless steel.
Because of the nature of the technique, physiological re-sponses such as the myogenic response can be measured. Data such as vessel wall thickness, changes in vessel and lumen diameter can be collected.

The experimental chamber is made of stainless steel and contains a window at the base of the chamber, allowing the vessel to be digitized for dimension analysis. The adjoining removable chamber is used for freezing or fixation. Rapid freezing or fixation is achieved by simply raising the seg-ment holder with the mounted vessel to the POM chamber, which takes less than a second.
This chamber can be included as part of a complete set-up or can be an add-on chamber to the 110P system.

The Acquisition & Analysis Package
Upright Zeiss or Nikon microscope (contact DMT for further specifications) with USB camera, PC and MyoVIEW data ac-quisition software.

SYSTEM

SPECIFICATIONS

  • Vessel size:  > 60 μm
  • Vessel alignment:  Manually X,Y & Z settings
  • Mounting supports: Glass pipettes
  • Chamber:  Dual, one nornal chamber and a composite chamber for freezing/ fixation
  • Chamber volumes: Max 10 ml
  • Chamber material:  Acid-resistant s-steel and POM
  • Chamber cover: With pipes for suction/gassing/superfusion
  • Pressure range:  0-250 mmHg (requires external pressure source. app. 1 ATO.)
  • Pressure calibration: Manual
  • Pressure resolution: 0.1 mmHg
  • Heating:  Built-in
  • Temp.range: Ambient - 50°C
  • Temp. resolution:  0,1°C
  • Temp. probe:  Included
  • Weight calibration:  Semi-automatic
  • Analogue output:  0-1.0V full scale (12 bit)
  • Digital output:   Serial interface - RS232/RS485
  • Voltage:  100 to 240 VAC (auto) 50/60 Hz
  • Ambient temp.: 15-30°C 
  • Fluid capacity:   250 ml

Optional accessories:

•    FlowMeter - 162FM
•    Enable pH meter on the interface
•    Peristaltic pump
•    Inverted Microscope
•    USB digital camera
•    MyoVIEW Data Acquisition Software
•    Vacuum pump
•    Electronic vacuum valve
•    Waste bottle
•    Gas supply manifold
•    Computer
•    Dissection tool kit
•    Disection microscope

 

banner_allentown_cz.jpg