Cell viability analyzer and cell counter NucleoCounter® NC-3000™

 Image cytometry as precise as flow cytometry. Cell viability analyzer and cell counter NucleoCounter NC-3000

Cell viability analyzer and cell counter NucleoCounter® NC-3000™

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An advanced image cytometer utilizing fluorescence imaging to characterize cell properties. The NC-3000™ can perform high speed cell count and viability determination and includes plug-and-play analytical assays of mammalian, yeast and insect cells. Pre-defined assays include a unique 5-minute cell cycle assay and a selection of apoptosis assays.

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Cell viability analyzer and cell counter NucleoCounter® NC-3000™

With the FlexiCyte™ license, the user can create protocols choosing light source, emission filters and detailed settings. The accompanying intuitive software can be used for data analysis of the results and has unlimited licenses.
The NC-3000™ benchtop image cytometer ensures flow-like analysis with high reproducibility and is maintenance and calibration free.

Key features:

  • No rinsing, clogging or maintenance
  • Easy to operate – no warm-up or calibration
  • CFR 21 part 11 ready
  • Automated PDF reports
  • Unlimited software licenses and free software updates
  • Excellent support by field application specialists
  • High-speed cell counting and advanced cell analysis
  • Visual inspection of cells – plot to image
  • Images are saved automatically and can be re-analyzed later
  • Low sample volume
  • Build your own assays with up to 4 analytical channels
  • Export data to FCS Express, FlowJo, etc.

Flexible methods for determination of viability and cell count

One-step viability and cell count without any pre-treatment

Simply load the cell suspension into the Via1-Cassette™ for viability and cell count determination. The inside of the Via1-Cassette™ is coated with the two fluorescent dyes acridine orange and DAPI automatically staining the total and dead cell populations, respectively.
The Via1-Cassette™ is volume calibrated ensuring high precision viability and cell count determination.

High speed viability and cell count of small sample volumes

The multi-chamber A8 slide™ enables high speed viability and cell count determinations of insect and mammalian cells with 8 counts in less than 3 minutes. Simply mix cell suspension with fluorescent dyes, load the chamber and press run.
ChemoMetec offers optimized counting and viability protocols for even aggregated cells or cells growing on microcarriers or in sphere cultures.


When counting manually the scientist can visually verify the counting. This verification of the count can likewise be performed with the intuitive NucleoView™ software included with the NC-3000™. Scatterplots and plots are interlinked making it possible to verify cell populations visually.

 

 

 

The accompanying NucleoView™ software allows for easy coupling between the obtained image and plots showing fluorescence for precise determination of the events to count.

2-step cell cycle

Cell cycle analysis is the most fundamental method to establish anomalies in cell division and thereby symptoms of cell damage. With ChemoMetec’s unique 2-step cell cycle a cell cycle can be performed in 5 minutes including treatment of cells! A cell cycle profile will readily be displayed in the accompanying PlotManager software and percentage or numbers of events in G0/G1, S-phase, G2 and sub-G1 can be identified.

2-step cell cycle assay of untreated and camptothecin-treated (CPT) Jurkat cells. Histograms displaying DNA-intensity (DAPI) can be used to define events in G0/G1, S-phase, G2 and sub-G1.

2-step cell cycle assay of untreated and camptothecin-treated (CPT) Jurkat cells. Histograms displaying DNA-intensity (DAPI) can be used to define events in G0/G1, S-phase, G2 and sub-G1.

Apoptosis assays

The NucleoCounter® NC-3000™ has 5 plug-and-play apoptosis assays: Mitochondrial Potential Assay, Annexin V Assay, Caspase 3/7, 8 & 9, Cell Vitality Assay and DNA fragmentation Assay. Covering early to late stages of apoptosis, these assays will allow for detailed study of progress of apoptosis in mammalian cells. With the user friendly accompanying PlotManager software, advanced data analysis can effortlessly be performed. The unique possibility to link between images, histograms and scatterplots allows for detailed and precise data analysis.

Viability and analysis of GFP transfected cells

Green Fluorescent Protein (GFP) is frequently used as a marker for transfection both in research and production. It is extremely important to determine the effect of transfection on cell viability. With the NucleoCounter® NC-3000™ plug-and-play GFP transfection assay, the number of viable and dead GFP-transfected cells can be determined with high precision. With the user-adaptable module FlexiCyte™, transfection percentage of cells expressing xFP-variants such as RFP or YFP can also easily be determined.

All cells are located using the DNA stain Hoechst 33342. GFP-transfected cells have green fluorescense whereas PI-stained dead cells will be red. All channels can be viewed on image individually (A-C) or as overlay (D), and analyzed cells can be shown (E). Detailed data analysis can be performed with the accompanying PlotManager software.

NC-3000™ GMP and 21 CFR Part 11-Ready

The NucleoCounter® NC-3000™ is ideal for the regulated QC, production and even PD and R&D departments. Instrument and method verification e.g. in combination with 21 CFR Part 11 ensures that the instrument performs and lives up to the requirements and demands of the pharmaceutical and biotech industries. Standardized pdf-reports, user control and registration of audit trails allow for documentation of all procedures. Combined with high precision and limited user influence this makes the NucleoCounter® NC-3000™ a one-of-a-kind cytometer.

User adaptable protocols with FlexiCyte™

The FlexiCyte™ module enables users to perform advanced cell analysis of a broad range of biomarkers and fluorescent proteins in mammalian cells. The combination of LEDs from UV to far red with a carefully selected set of emission filters allows for detection of a range of fluorescent biomarkers. The ‘Protocol Adaptation Wizard’ guides the user through selection of optimal settings. After image acquisition cell data are presented in the PlotManager as either scatter plots, histograms or both. Detailed data analysis can then be performed by linkage between images and plots.

Possible LED and emission filter combinations in the FlexiCyte™ module for NC-3000™.

2015    2014    2013    2012    2011   

 

 

2015

Increasing efficiency of human mesenchymal stromal cell culture by optimization of microcarrier concentrationand design of medium feed

Chen AK, Chew YK, Tan HY, et al.
Cytotherapy, 2015 Feb;17(2):163-73

Cell type: Mesenchymal Stem Cell (MSC), Stem Cell, Stromal Vascular Fraction (SVF)      Field: Cell Therapy


C5-curcuminoid-4-aminoquinoline based molecular hybrids: design, synthesis and mechanistic investigation of anticancer activity

Shamseer Kulangara Kandi, Sunny Manohar, Christian E. Vélez Gerena, et al.
New Journal of Chemistry, 2015,39, 224-234

Cell Type: N/A      Field: Cancer Research


 

 

2014

Dynamic rearrangement of F-actin is required to maintain the antitumor effect of trichostatin A.

Yang DH, Lee JW, Lee J, Moon EY.
PLoS One, 2014, May 20;9(5):e97352

Cell Type: HeLa      Field: Cell Biology


Association of nuclear-localized Nemo-like kinase with heat-shock protein 27 inhibits apoptosis in human breastcancer cells

Shaw-Hallgren G, Chmielarska Masoumi K, Zarrizi R, et al.
PLoS One, 2014, Mar 4;9(3):e90533

Cell Type: N/A      Field: Cell Biology


Polish natural bee honeys are anti-proliferative and anti-metastatic agents in human glioblastoma multiformeU87MG cell line.

Moskwa J, Borawska MH, Markiewicz-Zukowska R, et al.
PLoS One, 2014, Mar 4;9(3):e90533

Cell Type: U87MG      Field: Cancer Research


Age influence on stromal vascular fraction cell yield obtained from human lipoaspirates

Dos-Anjos Vilaboa S, Navarro-Palou M, Llull R.
Cytotherapy, 2014 Aug;16(8):1092-7

Cell Type: Stem Cell, Stromal Vascular Fraction (SVF)      Field: Cell Therapy


A new system for quality control in hematopoietic progenitor cell units before reinfusion in autologoustransplant

Scerpa MC, Rossi C, Daniele N, et al.
Transfusion, 2014 Mar;54(3):522-31

Cell Type: HPC-A      Field: Immunology
Assay: Mitochondrial potential, Vitality


Conjoint propagation and differentiation of human embryonic stem cells to cardiomyocytes in a definedmicrocarrier spinner culture

Lam AT, Chen AK, Li J, et al.
Stem Cell Research & Therapy, 2014 Sep 15;5(5):110

Cell Type: Embryonic cells, ESC, Stem Cell      Field: Stem Cell Research
Assay: Cell count & viability


Investigation of the cytotoxic, genotoxic, and apoptosis-inducing effects of estragole isolated from fennel(Foeniculum vulgare)

Villarini M, Pagiotti R, Dominici L, et al.
Journal of Natural Products, 2014 Apr 25;77(4):773-8

Cell Type: HepG2      Field: Cell Biology


Toxic effects of xylazine on endothelial cells in combination with cocaine and 6-monoacetylmorphine

Silva-Torres LA, Vélez C, Lyvia Alvarez J, et al.
Toxicology in Vitro, 2014 Oct;28(7):1312-9

Cell Type: HUVEC      Field: Toxicology


Plant-Derived Flavones as Inhibitors of Aurora B Kinase and Their Quantitative Structure-Activity Relationships

Jung Y, Shin SY, Yong Y, et al.
Chemical Biology & Drug Design, 2014 Oct 8

Cell Type: N/A      Field: Cell Biology


Apoptosis induced by microwave radiation in pancreatic cancer JF305 cells

Zhu W, Zhang W, Wang H, et al.
Canadian Journal of Physiology and Pharmacology, 2014 Apr;92(4):324-9

Cell Type: JF305      Field: Cancer Research


RAD001 enhances the radiosensitivity of SCC4 oral cancer cells by inducing cell cycle arrest at the G2/Mcheckpoint

Yu CC, Hung SK, Liao HF, et al.
Anticancer Research, 2014 Jun;34(6):2927-35

Cell Type: Cancer Cell      Field: Cancer Research


Xylazine as a drug of abuse and its effects on the generation of reactive species and DNA damage on humanumbilical vein endothelial cells

Silva-Torres L, Veléz C, Alvarez L, Zayas B.
Journal of Toxicology, 2014;2014:492609

Cell Type: HUVEC      Field: Toxicology


 

 

2013

Alkaline Phosphatase ALPPL-2 Is a Novel Pancreatic Carcinoma-Associated Protein

Dua P, Kang HS, Hong SM, et al.
Cancer Research, Mar 15;73(6):1934-45

Cell Type: Pancreatic cells      Field: Cancer Research
Assay: FlexiCyte™


The octadecaneuropeptide ODN prevents 6-hydroxydopamine-induced apoptosis of cerebellar grnule neurons through a PKC-MAPK-dependent pathway

Kaddour H, Hamdi Y, Vaudry D, et al.
Journal of Neurochemistry, 2013 May;125(4):620-33

Cell Type: Neurons      Field: Neurobiology
Assay: Vitality


Influence of vitamin D on cisplatin sensitivity in testicular germ cell cancer-derived cell lines and in a NTera2 xenograft model

Jørgensen A, Blomberg Jensen M, Nielsen JE, et al.
Journal of Steroid Biochemistry and Molecular Biology, 2013 Jul;136:238-46

Cell Type: Cancer Cell      Field: Cancer Research
Assay: Cell count & viability


Metallic gold beads in hyaluronic acid: a novel form of gold-based immunosuppression? Investigations of the immunosuppresive effects of metallic gold on cultured J774 macrophages and on neuronal gene exrpession in experimental autoimmune encephalomyelitis

Pedersen DS, Tran TP, Smidt K, et al.
Biometals, 2013 Jun;26(3):369-85

Cell Type: Macrophages      Field: Neurobiology
Assay: Vitality


Structural Properties of Polyphenols Causing Cell Cycle Arrest at G1 Phase in HCT116 Human Colorectal Cancer Cell Lines

Shin SY, Yoon H, Ahn S, et al.
International Journal of Molecular Sciences, 2013 Aug 19;14(8):16970-85

Cell Type: HCT116      Field: Cancer Research
Assay: Cell cycle


Chromenylchalcones showing cytotoxicity on human colon cancer cell lines and in silico docking with aurora kinases

Shin SY, Yoon H, Ahn S, et al.
Bioorganic & Medicinal Chemistry, 2013 Jul 15;21(14):4250-8

Cell Type: HCT116      Field: Cancer Research
Assay: Annexin V, Vitality


RNA Interference-Mediated Simultaneous Silencing of Four Genes Using Cross-Shaped RNA

Lee TY, Chang CI, Lee D, et al.
Molecules and Cells, 2013 Apr;35(4):320-6. doi: 10.1007/s10059-013-2316-7

Cell Type: HeLa      Field: Cell Biology
Assay: FlexiCyte™


Chemosensitivity Induced by Down-regulation of MicroRNA-21 in Gemcitabine-resistant Pancreatic Cancer Cells by Indole-3-Carbinol

Paik WH, Kim HR, Park JK, et al.
Anticancer Research, 2013 Apr;33(4):1473-81

Cell Type: Cancer Cell      Field: Cell Biology
Assay: Cell cycle


Resveratrol Inhibits Alpha-Melanocyte-Stimulating Hormone Signaling, Viability, and Invasiveness in Melanoma Cells

Chen YJ, Chen YY, Lin YF, et al.
Evidence-Based Complementary and Alternative Medicine, 2013:632121

Cell Type: Melanoma cells      Field: Cancer Research
Assay: FlexiCyte™


Image cytometer method for automated assessment of human spermatozoa concentration

Egeberg DL, Kjaerulff S, Hansen C, et al.
Andrology, 2013 Jul;1(4):615-23

Cell Type: Sperm cells      Field: Reproduction
Assay: Cell count & viability


 

 

2012

Mitochondrial fragmentation caused by phenanthroline promotes mitophagy

Park SJ, Shin JH, Kim ES, et al.
FEBS Letters, 2012 Dec 14;586(24):4303-10

Cell Type: N/A      Field: Cell Biology
Assay: Mitochondrial potential


The influence of the number of cells scored on the sensitivity in the comet assay

Sharma AK, Soussaline F, Sallette J, Dybdahl M.
Mutation Research, 2012 Dec 12;749(1-2):70-5

Cell Type: N/A      Field: Toxicology
Assay: Cell count & viability


Ultraviolet C Irradiation Induces Different Expression of Cyclooxygenase 2 in NIH 3T3 Cells and A431 Cells: The Roles of COX-2 Are Different in Various Cell Lines

Tai MH, Weng CH, Mon DP, et al.
International Journal of Molecular Sciences, 2012;13(4):4351-66

Cell Type: 3T3, A431      Field: Cancer Research
Assay: DNA fragmentation


Sertindole, a potent antagonist at dopamine D₂ receptors, induces autophagy by increasing reactive oxygen species in SH-SY5Y neuroblastoma cells

Shin JH, Park SJ, Kim ES, et al.
Biological and Pharmaceutical Bulletin, 2012;35(7):1069-75

Cell Type: SH-SY5Y      Field: Cell Biology
Assay: Cell count & viability


Kinetics of DNA methylation inheritance by the Dnmt1-including complexes during the cell cycle

Hervouet E, Nadaradjane A, Gueguen M, et al.
Cell division, 2012, Feb 20;7:5

Cell Type: N/A      Field: N/A
Assay: Cell cycle


 

 

2011

A receptor tyrosine kinase inhibitor, Tyrphostin A9 induces cancer cell death through Drp1 dependent mitochondria fragmentation

Park SJ, Park YJ, Shin JH, et al.
Biochemical and Biophysical Research Communications, 2011 May 13;408(3):465-70

Cell Type: N/A      Field: Cell Biology
Assay: Mitochondrial potential


Proximity ligation in situ assay for monitoring the global DNA methylation in cells

Hervouet E, Hulin P, Vallette FM, Cartron PF.
BMC Biotechnology, 2011 Apr 6;11:31

Cell Type: N/A      Field: N/A
Assay: Cell cycle


Niclosamide induces mitochondria fragmentation and promotes both apoptotic and autophagic cell death

Park SJ, Shin JH, Kang H, et al.
BMB reports, 2011 Aug;44(8):517-22

Cell Type: N/A      Field: Cell Biology
Assay: Mitochondrial potential