Culture-insert 4 well

Culture-insert 4 well


4 well silicone insert with 4 defined cell-free gaps and a center migration area, suitable for wound healing, migration assays, 2D invasion assays, and co-cultivation of cells.

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Culture-insert 4 well

  • Complete solution for wound healing experiments, requiring only a few steps from sample preparation to image analysis
  • Reproducible experiments owing to: four defined 500 µm cell free gaps and one 1000 µm center gap, no leakage during cultivation, and no material being left behind after the insert’s removal
  • Suitable for 4 experiments, including a center gap that allows to observe the migration of all cell types


  • Wound healing assays 
  • Migration assays 
  • 2D invasion assays 
  • Co-cultivation of cells

Principle for wound healing and migration assays

Step 1: cell seeding and attachment

Step 2: remove insert after cultivation

Step 3: overlay patches with culture medium

Various applications

Wound healing / Migration

The Culture-Insert 4 Well allows for wound healing and migration assays with four cell-free gaps with a 0.5 mm gap width, and one center area with a 1 mm diagonal distance. Compared to the 2 well version, more cells can be analyzed and a higher throughput is realized. Two cell-free gaps create a technical replicate in the very same well.

Two conditions of wound healing / Migration

With the Culture-Insert 4 Well, a wound healing experiment and a control experiment can be done in the same well. The control cells can be pre-treated with any kind of treatment (e.g., drugs, plasmids, mRNA, or virus particles).

Four Conditions for co-cultivation / Invasion

By seeding up to four different cell types, the invasiveness of adherent cells can be analyzed. Non-invasive cell types can be cultured next to each other, sharing the same medium. This co-culture allows the cells to communicate and share factors for differentiation studies or long-term culturing.

Invasion and co-cultivated cells can be imaged by unsing high-resolution fluorescence microscopy.


Number of wells 4
Outer dimensions Ø 17 mm
Volume per well 110 µl
Growth area per well 0,35 cm2
Coating area per well 1,23 cm²

Width of cell free gap

- Two cell fronts

- Four cell fronts (center)


500 µm +/- 50 µm

1000 µm +/- 100 µm

Material Biocompatible silicone
Bottom No bottom - sticky underside

Ordering information

Cat. no. Description Pcs./Box
80466 Culture-Insert 4 Well in µ-Dish 35 mm, high ibiTreat: ready to use, tissue culture treated, sterilized 30
80469 25 Culture-Inserts 4 Well for self-insertion: in a 10 cm transport dish, sterilized 25