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ibidi Heating system, universal fit

ibidi Heating system, universal fit

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Heating System for all live cell imaging applications including high resolution, TIRF, and confocal microscopy.

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ibidi Heating system, universal fit

  • Compatible with all microscopy platforms - fits every inverted microscope that has a frame or holder for 96 well plates
  • Excellent illumination of the sample - no condensation due to heated lid, optimal magnetic sample stabilization, and suitable for DIC
  • Perfectly suited for live cell imaging applications - full incubator conditions on the microscope
  • Ideal for use with motorized stages when used with the Heated Plate for 4 µ-Slides
ibidi Temperature controller Heated plate in
multi-well format
Heated glass lLid,
universal fit
Heating insert for ibidi
and non-ibidi slide formats

Applications

  • Live cell imaging that requires incubator conditions on the microscope, for example:
    - Chemotaxis
    - Angiogenesis
    - Cell migration, proliferation, wound healing
    - Cell culture under flow
  • Imaging applications with extra focus stability, for example:
    - TIRF (Total Internal Reflection Fluorescence)
    - Confocal microscopy
  • Anaerobic conditions or oxidative stress

Specifications

Temperature controller   Heated plate and lid
Environmental humidity Relative humidity
0% - 99%
  Dimensions Plate / Lid
(in mm)
127,5 x 85,5 x 8 /
135 x 85,5 x 20,5
Environmental
temperature
+5°C - +40°C
(50% rel. humidity)
  Height of the system 26,5 mm
Dimensions
(H x W x D in mm)
90 x 170 x 230   Weight of incubation chamber with insert 330 g
Weight 3,4 kg   Gas inlet Luer connector (M5 thread) with luer lock
Input voltage 230 V / 50 Hz
115 V / 60 Hz
  Restrictions for objective lenses None
      Custom openings On request

Heating stages for live cell imaging

The independently controlled, heated glass lid of the ibidi Heating & Incubation system solves the problem of condensation in live cell imaging.

By heating the lid to a temperature higher than the plate, a vertical temperature gradient is created. This gradient and an active humidity control prevent the formation of condensation on the lid of the Petri dish. The temperature at the cells’ site is maintained at a constant 37 °C.

Components of the ibidi Heating & incubation system, universal fit

ibidi Temperature controller

  • Provides excellent temperature stability within the incubation chamber
  • Four control channels allow to connect a heated plate, a heated lid, and two additional heated components (e.g., an objective heater)

Technical features

  • Temperature stability: ± 0.05 °C
  • Temperature uniformity across the insert: ± 0.5 °C
  • Temperature range: Room temperature - 45 °C
  • Control channels: 4
  • TempControl software for remote control and data recording included
  • External temperature sensor included
  • Adjustable alarm function included
  • Analog output for extreme noise reduction optional

Heated plate in a multi-well format for 1 chamber

  • Can be used with any inverted microscopy system
  • Fits into both manual and automated stages
  • Can be placed into any regular multi-well frame that comes with your microscope
  • Adaptable to all types of microscopy chambers, such as µ-Slides and µ-Dishes plus non-ibidi formats

Heated plate in a multi-well format for 4 µ-Slides

  • Can be used with any inverted microscopy system
  • Ideal for use with motorized stages
  • Can be placed into any regular multi-well frame that comes with your microscope
  • Compatibe with all ibidi µ-Slides and non-ibidi slide formats

Heated glass lid, universal fit, for use with CO2 and humidity

  • For use with CO2, O2, and humidity to provide a complete environmental chamber
  • Independently controlled glass lid, warmed to 40 °C, prevents the formation of condensed water on the lid of the microscopy chamber or plate
  • No scattering of light or shadow formation

Heating inserts

  • For all ibidi µ-Slides
  • For µ-Dish 35mm. high
  • For µ-Dish 35mm. low
  • For LabTek TM chambered coverglass
  • To be used with the Heated Plate in Multi-Well Format for 1 chamber

Heating insert adapter for perfusion assays

  • To be used with the ibidi Pump system for live cell imaging under flow conditions

Gas mixer for the incubation unit

The heated lid also comes with a gas inlet. The ibidi Gas incubation system upgrades the ibidi Heating system to a complete stage top incubator for all live cell imaging applications, including high resolution, TIRF, and confocal microscopy.

The ibidi Gas incubation system is suitable for various experimental conditions (e.g., pH or hypoxia). It is available in two versions: Gas mixer for CO2, Gas mixer for CO2 and O2.

Overview of the heating and incubation system, universal fit

 

The connecting possibilities to other ibidi systems

ibidi Heating system for temperature control of multi-well plates

ibidi has developed a Heating System for the use with K-Frame Stages or the Nikon Ti-S-E and Ti-S-ER Motorized Stage which hold multi-well plates. Thus, this system permits the user to control the temperature of samples held in multi-well plates.

 

Live cell imaging under flow conditions

The ibidi Heating & Incubation System can be adapted for live cell imaging under flow conditions. Connect the ibidi Pump System with the ibidi Heating System, Universal Fit, by using the Heating Insert Adapter for perfusion assays. This creates the perfect environment for long-term cell studies (several days) under flow conditions.

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Manufacturer:
Heating system for K-Frame stages or the Nikon Ti-S-E and Ti-S-ER motorized stage which hold multi-well plates (for low magnification only).
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Manufacturer:
A pump system for the cultivation of cells under flow for simulation of blood vessels.
Add to request
Manufacturer:
A gas mixer that upgrades the ibidi Heating System to a complete stage top incubator for all live cell imaging applications with CO2, O2, and humidity control.