Szukaj

Immunofluorescence chambers

Immunofluorescence chambers

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Removable silicone cultivation chambers for cell culture and immunofluorescence staining.

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Immunofluorescence chambers

  • Perfect for immunofluorescence staining and long-term sample storage
  • Cost effective classic immunofluorescence assays
  • Large growth area combined with a small staining volume (especially 3 Well Chamber, removable)
  • Suitable for use with upright and inverted microscopes

Applications

  • Immunofluorescence assays using standard coverslip techniques
  • Long-term sample storage
  • 3, 8, or 12 fold sample preparation of adherent cells and tissue samples

Technical features

  • Self-adhesive silicone gasket mounted on a standard glass slide
  • Biocompatible silicone material
  • Non-fluorescent glass slide with frosted ends for easy handling and labeling
  • Suitable for use with upright and inverted microscopes, after staining and mounting with a coverslip
  • Staining volume reduction by glass coverslip in 3 Well Chamber, removable
  • Compatible with all common mounting media
  • Optional: The gasket may be transferred to any flat clean surface for cell cultivation

Standard procedure 3 well | 8 well | 12 well chamber, removable

Low volume staining procedure 3 well chamber, removable

Staining protocol

Specification

Features
Number of wells 3 8 12
Dimensions of wells (w x l x h) in mm 16,5 x
16,5 x 8,0
12 x
7,75 x 8,0
7,5 x
7,5 x 8
Volume per well 1100 µl
(*150 µl)
400 µl 250 µl
Growth area per well 1,66 cm² 0,93 cm² 0,56 cm²
Coating area per well 3,73 cm² 2,63 cm² 1,90 cm²
Material Biocompatible silicone
Bottom Glass slide (26 x 76 x 1 mm)

* Volume reduction for staining when using a 15 mm glass coverslip (Cat. No. 10815)

Ordering information

Cat. no. Description Pcs./Box
80381 3 Well Chamber, removable: microscopy glass slide, sterilized 15
80841 8 Well Chamber, removable: microscopy glass slide, sterilized 15
81201 12 Well Chamber, removable: microscopy glass slide, sterilized 15
10811 Coverslips for chambers, removable: No. 1.5H (170 µm +/- 5 µm) D 263 M Schott glass , 24 mm x 60 mm, unsterile 100
10815 Coverslips and coverslip pick-up tool for 3 well chamber, removable: # 1.5H (170 µm+/- 5 µm) D 263 M Schott glass, Ø 15 mm, unsterile, including one silicone rubber tool for convenient glass coverslip handling 100