Indirect gas calorimetry module CaloSys

Indirect gas calorimetry module CaloSys


Module for calorimetric measurment with gas&temeprature sensors and software to perform experiments. Dedicated for use in metabolic analysis mice and rats during f.e. feeding, efficiency, motor function studies. Fully compatible with PhenoMaster system.

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  • Description
  • Publications

Indirect gas calorimetry module CaloSys

Indirect gas calorimetry uses measurements of the animal’s oxygen consumption (VO2) and carbon dioxide production (VCO2) to estimate various metabolic parameters, including the respiratory exchange rate (RQ), energy expenditure (EE), substrate utilization (fat reserves vs. carbohydrate catabolism), and more.

The gas content of an air sample that has been drawn from a test cage is analyzed by a gas sensor unit consisting of one oxygen and one carbon dioxide gas sensor. In a continuous CaloSys setup every cage has its dedicated gas sensor unit ensuring immediate analysis at high temporal resolution (in the range of seconds). If such high temporal resolution is not required, as for most calorimetric studies, CaloSys can be operated in a more economical multiplexed setup, where two or more cages share a common sensor unit – gas samples are analyzed successively from one cage after the other. In a multiplexed setup, the temporal resolution lies in the range of minutes and is directly dependent on the number of cages per sensor unit. To speed up sampling cycles, multiplexed setups can optionally be equipped with high-speed gas sensor units.

The high-speed gas sensors are a paramagnetic differential O2 sensor and an infrared spectroscopic dual beam CO2 sensor. Both are maintenance free and are periodically automatically calibrated under software control using 3 reference gases from a bottle.

The PhenoMaster module for indirect gas calorimetry (CaloSys) is a fully automated high-throughput system for short- and long-term metabolic experiments offering the best possibilities for PULL and PUSH, continuous and multiplexed respirometry.
The unique advantage: CaloSys is highly versatile!
It can be used with a home cage setup granting maximal animal welfare and various combination options with other modules, but it can also be integrated into:

Key features

  • calorimetry in the home cage - low stress, high animal wellfare
  • pull and push air flow principle
  • continuous or multiplexed sampling
  • optional high-speed gas sensors accelerate multiplexed measurements
  • an auto-calibration unit facilitates sensor adjustment prior to experiment
  • programmable mass-flow controllers for every cage optimize the flow rate according to animal size/species
  • every cage is equipped with a high-end temperature sensor for enhanced measurement accuracy
  • the variable influence of water is eliminated from gas measurements by a first pass peltier-based gas drying unit prior to measuring oxygen or carbon dioxide
  • exercise calorimetry on the CaloTreadmill or the CaloWheel
  • calorimetry under thermoneutral or rapid temperature challenge conditions in the Climate Chamber
  • modular design allows simultaneous assessment of metabolic, behavioral, cognitive, and physiological parameters
  • PhenoMaster Software operates a virtually unlimited number of test systems simultaenously without experimenter interference - ideal for high-throughput experiments
Al-Ahad Sabbagha NGA, Kao HJ, Yang CF, Huang CC, Lin WD, Tsai F, Chen TH, Tarn WY, Wu JY, Chen YT
Alternative splicing in acad8 resulting a mitochondrial defect and progressive hepatic steatosis in mice
Pediatr Res 2011; 70(1):31-6
PhenoMaster / LabMaster (Drinking / Feeding, Calorimetry)
Haemmerle G, Moustafa T, Woelkart G, Büttner S, Schmidt A, van de Weijer T, Hesselink M, Jaeger D, Kienesberger PC, Zierler K, Schreiber R, Eichmann T, Kolb D, Kotzbeck P, Schweiger M, Kumari M, Eder S, Schoiswohl G, Wongsiriroj N, Pollak NM, Radner FPW, Preiss-Landl K, Kolbe T, Rülicke T, Pieske B, Trauner M, Lass A, Zimmermann R, Hoefler G, Cinti S, Kershaw EE, Schrauwen P, Madeo F, Mayer B, Zechner R
ATGL-mediated fat catabolism regulates cardiac mitochondrial function via PPAR-alpha and PGC-1
Nat Med 2011; 17(9):1076-85
PhenoMaster / LabMaster (Calorimetry)
Hollis JH, Jonaidi H, Lemus M, Oldfield BJ
The endocannabinoid Arachidonylethanolamide attenuates aspects of lipopolysaccharide-induced changes in energy intake, energy expenditure and hypothalamic Fos expression
J Neuroimmunol 2011; 233:127-34
PhenoMaster / LabMaster (Calorimetry, Metabolic PhenoCage, Drinking / Feeding, Activity)
Jamieson PM, Cleasby ME, Kuperman Y, Morton NM, Kelly PAT, Brownstein DG, Mustard KJ, Vaughan JM, Carter RN, Hahn CN, Hardie DG, Seckl JR, Chen A, Vale WW
Urocortin 3 transgenic mice exhibit a metabolically favourable phenotype resisting obesity and hyperglycaemia on a high-fat diet
Diabetologia 2011; 54:2392-403
PhenoMaster / LabMaster (Drinking / Feeding, Activity, Calorimetry)
Jordan SD, Krüger M, Willmes DM, Redemann N, Wunderlich FT, Brönneke HS, Merkwirth C, Kashkar H, Olkkonen VM, Böttger T, Braun T, Seibler J, Brüning JC
Obesity-induced overexpression of miRNA-143 inhibits insulin-stimulated AKT activation and impairs glucose metabolism
Nat Cell Biol 2011; 13(4):434-446
PhenoMaster / LabMaster (Calorimetry)
Li Y, Tong X, Rumala C, Clemons K, Wang S
Thrombospondin1 deficiency reduces obesity-associated inflammation and improves insulin sensitivity in a diet-induced obese mouse model
PLoS ONe 2011; 6(10):e26656
PhenoMaster / LabMaster (Calorimetry, Drinking / Feeding)
Lin HV, Ren H, Samuel VT, Lee HY, Lu TY, Shulman GI, Accili D
Diabetes in mice with selective impairment of insulin action in Glut4-expressing tissues
Diabetes 2011; 60, 3:700-708
PhenoMaster / LabMaster (Calorimetry, Feeding, Activity)
Ma T, Liaset B, Hao Q, Koefoed Petersen R, Fjære E, Thi Ngo H, Haukûs Lillefosse H, Ringholm S, Brask Sonne S, Thue Treebak J, Pilegaard H, Frøyland L, Kristiansen K, Madsen L
Sucrose counteracts the anti-inflammatory effect of fish oil in adipose tissue and increases obesity development in mice
PLoS ONE 2011; 6(6):
PhenoMaster / LabMaster (Calorimetry)
Nelson DW, Gao Y, Spencer NM, Banh T, Yen CLE
Deficiency of MGAT2 increases energy expenditure without high-fat feeding and protects genetically obese mice from excessive weight gain
J Lipid Res 2011; 52:1723-32
PhenoMaster / LabMaster (Drinking / Feeding, Calorimetry)
Pfluger PT, Castañeda TR, Heppner KM, Strassburg S, Kruthaupt T, Chaudhary N, Halem H, Culler MD, Datta R, Burget L, Tschöp MH, Nogueiras R, Perez-Tilve D
Ghrelin, peptide YY and their hypothalamic targets differentially regulate spontaneous physical activity
Physiol Behav 2011; 105:52-61
PhenoMaster / LabMaster (Drinking / Feeding, Activity, Calorimetry)
Ravussin Y, Gutman R, Diano S, Shanabrough M, Borok E, Sarman B, Lehmann A, LeDuc CA, Rosenbaum M, Horvarth TL, Leibel RL
Effects of chronic weight perturbation on energy homeostasis and brain structure in mice
Am J Physiol Regul Integr Comp Physiol 2011; 300:1352-62
PhenoMaster / LabMaster (Calorimetry, Activity)
Rossi J, Balthasar N, Olson D, Scott M, Berglund E, Lee CE, Choi MJ, Lauzon D, Lowell BB, Elmquist JK
Melanocortin-4 receptors expressed by cholinergic neurons regulate energy balance and glucose homeostasis
Cell Metab 2011; 13:195-204
PhenoMaster / LabMaster (Calorimetry)
Rousso-Noori L, Knobler H, Levy-Apter E, Kuperman Y, Neufeld-Cohen A, Keshet Y, Akepati VR, Klinghoffer RA, Chen A, Elson A.
Protein tyrosine phosphatase epsilon affects body weight by downregulating leptin signaling in a phosphorylation-dependent manner
Cell Metab 2011; 13:562-572
PhenoMaster / LabMaster(Calorimetry, ActiMot, Drinking / Feeding)
Rousso-Noori L, Knobler H, Levy-Apter E, Kuperman Y, Neufeld-Cohen A, Keshet Y, Akepati YR, Klinghoffer RA, Chen A, Elson A
Protein tyrosine phosphatase epsilon affects body weight by downregulating leptin signaling in a phosphorylation-dependent manner
Cell Metab 2011; 13:562-72
PhenoMaster / LabMaster (Drinking / Feeding, Activity, Calorimetry)
Scott MM, Williams KW, Rossi J, Lee CE, Elmquist JK
Leptin receptor expression in hindbrain Glp-1 neurons regulates food intake and energy balance in mice
J Clin Invest 2011; 121(6):2413-21
PhenoMaster / LabMaster (Drinking / Feeding, Activity, Calorimetry)
Steen Petersen P, Jin C, Nygaard Madsen A, Rasmussen M, Kuhre R, Egerod KL, Bo Nielsen L, Schwartz TW, Holst B
Deficiency of the GPR39 receptor os associated with obesity and altered adipocyte metabolism
FASEB J 2011; 25:1-12
PhenoMaster / LabMaster (Drinking / Feeding, Calorimetry)
Taschler U, Radner FPW, Heier C, Schreiber R, Schweiger M, Schoiswohl G, Preiss-Landl K, Jaeger D, Reiter B, Koefeler HC, Wojciechowski J, Theussl C, Penninger JM, Lass A, Haemmerle G, Rechner R, Zimmermann R
Monoglyceride lipase deficiency in mice impairs lipolysis and attenuates diet-induced insulin resistance
J Biol Chem 2011; 286(20):17467-77
PhenoMaster / LabMaster (Drinking / Feeding, Calorimetry)
Tauriainen E, Luostarinen M, Martonen E, Finckenberg P, Kovalainen M, Huotari A, Herzig KH, Lecklin A, Mervaala E
Distinct effects of calorie restriction and resveratrol on diet-induced obesity and Fatty liver formation
J Nutr Metab 2011; :525094
PhenoMaster / LabMaster (Calorimetry, Drinking / Feeding, Activity)
Vernia S, Heredia M, Criado O, Rodriguez de Cordoba S, Garcia-Roves PM, Cansell C, Denis R, Luquet S, Foufelle F, Ferre P, Sanz P
Laforin, a dual specificity phosphatase involved in lafora disease, regulates insulin response and whole-body energy balance in mice
Hum Mol Genet 2011; 20(13):2571-84
PhenoMaster / LabMaster (Drinking / Feeding, Activity, Calorimetry)
Veyrat-Durebex C, Poher AL, Caillon A, Montet X, Rohner-Jeanrenaud F
Alterations in lipid metabolism and thermogenesis with emergence of brown adipocytes in white adipose tissue in diet-induced obesity-resistant Lou/C rats
Am J Physiol Endocrinol Metab 2011; 300:1146-57
PhenoMaster / LabMaster (Drinking / Feeding, Activity, Calorimetry)
Wellner N, Tsuboi K, Madsen AN, Holst B, Diep TA, Nakao M, Tokumura A, Burns MP, Deutsch DG, Ueda N, Hansen HS
Studies on the anorectic effect of N-acylphosphatidylethanolamine and phosphatidylethanolamine in mice
Biochim Biophys Acta 2011; 1811:508-12
PhenoMaster / LabMaster (Drinking / Feeding, Activity, Calorimetry)
Wong KE, Kong J, Zhang W, Szeto FL, Ye H, Deb DK, Brady MJ, Chun Li Y
Targeted expression of human vitamin D receptor in adipocytes decreases energy expenditure and induces obesity in mice
J Biol Chem 2011; 286(39):33804-10
PhenoMaster / LabMaster (Drinking / Feeding, Activity, Calorimetry)