Pressure Myograph System 120CP

Confocal pressure myograph system - 120CP

Pressure Myograph System 120CP

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The Confocal Pressure Myograph System - 120CP is a system designCed specifically for use with laser scanning confocal microscopes (LSCM) and other high magnification microscopes for imaging studies where intracellular processes within the smooth muscle cells or endothelial cells of the intact, pressurized vessel are needed.

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  • Description

Pressure Myograph System 120CP

FEATURES & BENEFITS

  • Study the structure and function of small vessels >40 μm under near-physiological conditions
  • Specifically designed for use with laser scanning microscopes and imaging systems
  • Ideal for studying intracellular ion concentrations and isobaric constriction
  • Bath design enables easy access for aperture lenses
  • Designed to accommodate objective working distances as low as 100 μm

The Confocal Pressure Myograph System - 120CP is a system designCed specifically for use with laser scanning confocal microscopes (LSCM) and other high magnification microscopes for imaging studies where intracellular processes within the smooth muscle cells or endothelial cells of the intact, pressurized vessel are needed. This system is ideal for studying intracellular ion concentrations such as calcium or tracking the trafficking of fluorescent probes, tags or proteins in small vessels (internal diameter >40 μm). But the chamber can still be used for standard pressure myography. 
​The conical open bath design allows easy access for high magnification/high numerical aperture lenses used in inverted microscopes. It also allows access for direct immers​ion lenses used in upright confocal microscopy. In addition, the top part of the myograph can be easily lifted, facilitating maintenance and mounting.

Specially designed and angled mounting supports in combination with a z-axis manipulator facilitate precise vertical positioning of an isolated blood vessel directly above or on top of the chamber window, permitting the use of objectives with working distances as low as 100 μm on an inverted LSCM.
The intravascular pressure and the pressure gradient along the vessel can be precisely controlled. A built-in heating system maintains bath temperature above ambient temperature. The customized chamber cover includes ports for superfusion, for rapid draining and filling, and for gas bubbling. To facilitate cleaning, the chamber is made of acid-resistant stainless steel. When using the myograph on a confocal microscope, data acquisition of inlet and outlet pressure, force and temperature is possible by using either the analog outputs or the serial interface. 
 
Obtaining the NEW MyoVIEW data acquisition software, the Confocal Pressure Myograph System - 120CP can easily be used on a normal inverted light microscope for obtaining data such as vessel wall thickness, changes in vessel and lumen diameter, intravascular pressure, and a host of other calculated parameters, such as shear stress and vascular resistance.