Tyrosine hydroxylase (TH)-Cre rat

Tyrosine hydroxylase (TH)-Cre rat

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Model with expresion of Cre recombinase. For applications in optogenetic, expression and nockout recombined genes and DREADD.

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  • Description
  • Publications

Tyrosine hydroxylase (TH)-Cre rat

Nomenclature: SD- Th-cre tm1sage

Genotype: Homozygous

Description: This model expresses cre-recombinase under the control of the endogenous tyrosine hydroxylase promoter enabling specific expression in dopaminergic neurons. This model possesses a targeted insertion of (IRES)-cre immediately after the translational stop in the open reading frame of Th. The TH-Cre rat is useful for applications requiring tissue specific expression, including optogenetics and breeding with transgenic floxed lines.

Research applications

  • optogenetics
  • designer receptors exclusively activated by designer drugs DREADD
  • expression/knockout of floxed genes

Characteristics

  • Cre recombinase driven by endogenous TH promoter
  • no observed ectopic expression of cre
  • targeted insertion eliminates possible gene disruption that may occur in random insertion technologies such as BAC
  • background strain: Sprague-Dawley

Coat color: White, albino.

Strain code: TGRA8400

Bred in: US

Whole-rat conditional gene knockout via genome editing

Andrew J Brown,  Daniel A Fisher, Evguenia Kouranova, Aaron McCoy, Kevin Forbes, Yumei Wu, Rachel Henry, Diana Ji, Andre Chambers, Joe Warren, Weiguo Shu, Edward J Weinstein. Xiaoxia Cui
Nature Methods 10, 638–640 (2013) doi:10.1038/nmeth.2516