Szukaj

µ-Dish 35 mm, high ESS

µ-Dish 35 mm, high ESS

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A 35 mm imaging dish with an Elastically Supported Surface (ESS) used for growing cells under “in vivo-near” conditions.

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µ-Dish 35 mm, high ESS

  • “in vivo-near” cell culture on elastically supported surface
  • High resolution microscopy on soft cell culture surface
  • Based on the standard µ-Dish 35 mm, high

Applications

  • Cell culture under “in vivo-near” conditions, e.g. cardiomyocytes
  • Cellular mechanics research
  • Immunofluorescence stainings
  • Live cell imaging of cell differentiation on soft surface
  • Transfection
  • Suitable for DIC , when used with the special DIC Lid

Technical features

  • 40 μm thick PDMS (Polydimethylsiloxane) layer on top of a 100 μm thick glass cover
  • Available stiffness: 1.5 kPa, 15 kPa, and 28 kPa
  • Biocompatible soft cell culture material
  • High walls with a standard height for easy handling
  • Lid with lock position, which minimizes evaporation
  • Rim for easy opening
  • No autofluorescence
  • Fully compatible with the ibidi Heating System

 

Differentiated mouse fibroblasts cultured on elastic surface (ESS 28 kPa)

  • green: zyxin (Alexa 488 conjugated antibody)
  • red: alpha- smooth muscle actin (Cy5 conjugated antibody)

(courtesy R. Merkel, Jülich, Germany)

Specification

Features
Ø µ-Dish 35 mm
Volume high / low 2 ml
Growth area 3,5 cm2
Coating area using 400 µl 4,1 cm2
Ø observation area 21 mm
Height with lid high / low 14 / 12 mm
Bottom elastic surface (40 µm) on
glass coverslip (100 µm)

Ordering information

Cat. no. Description Pcs./Box
81291 µ-Dish 35 mm, high, ESS 1.5 kPa, Uncoated: Ø 35 mm, high wall (2 ml volume), elastic surface with a stiffness of 1.5 kPa, hydrophobic, sterilized 30
81391 µ-Dish 35 mm, high, ESS 15 kPa, Uncoated: Ø 35 mm, high wall (2 ml volume), elastic surface with a stiffness of 15 kPa, hydrophobic, sterilized 30
81191 µ-Dish 35 mm, high, ESS 28 kPa, Uncoated: Ø 35 mm, high wall (2 ml volume), elastic surface with a stiffness of 28 kPa, hydrophobic, sterilized 30
81199 µ-Dish 35 mm, high, ESS Variety Pack, Uncoated: Ø 35 mm, high wall (2 ml volume), elastic surface with a stiffness of 1.5, 15, and 28 kPa, hydrophobic, sterilized 30