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µ-Slide chemotaxis, polymer bottom

µ-Slide chemotaxis, polymer bottom

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Used to investigate chemotaxis of fast or slow migrating adherent cells and non-adherent cells in gel matrices.

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µ-Slide chemotaxis, polymer bottom

  • Chemotaxis measurement in real-time
  • Stable gradients for long-term experiments
  • Reproducible results with reliable and user-independent data

Applications

  • Chemotaxis of neutrophiles, lymphocytes, and monocytes
  • 3D chemotaxis of leukoycytes or cancer cells in ECM-like matrix
  • Chemotaxis experiments of fast or slow migrating cells
  • Invasion assays of tumor cells in Matrigel™

Technical features

  • Chamber geometry optimized for cells in 3D matrices
  • 3 chambers on one slide for parallel assays
  • Ideal for collagen gels, hydrogels, Matrigel™, or similar aqueous gels
  • Ready to use, i.e., no assembly required
  • Lettered and numbered chambers and reservoirs

Application examples

Cells, embedded into a gel, migrating towards a chemoattractant.

Dendritic cell (mouse) with LifeAct-labeled F-actin that is migrating towards CCL 19 inside a Collagen I gel matrix. Spinning disc confocal microscopy (objective lens 63 x).

HT-1080 cells migrating towards FCS inside a gel matrix (objective lens 10 x).

Data analysis

1. Chemotaxis and Migration Tool

To facilitate analysis of results from chemotaxis experiments (time stacks) ibidi has developed this software analysis tool. The tool provides various types of graphs and statistical tests for advanced analysis of experimental data.

2. Automated quantitative chemotaxis image analysis - WimTaxis

Manual acquisition and evaluation of 2D cell migration is time consuming and lacks in objectivity. Wimasis, the certified partner of ibidi for quantitative image analysis, developed an analysis solution to quantitatively evaluate 2D chemotaxis and migration. The Image Analysis solution WimTaxis generates fast results, without the need for extra hardware or software.

Specification

Features
Chemotaxis chambers on slide 3
Volume per chamber 120 µl
Observation area 2 x 1 mm²
Coating area per chamber
- When coating full chamber
- When coating observation area only

3,50 cm2
0,27 cm2
Distance between chambers 18,5 mm
Total height with plugs 12 mm
Volume chemoattractant 30 µl
Bottom ibidi Polymer Coverslip

Ordering information

Cat. no. Description Pcs./Box
80326 µ-Slide chemotaxis, ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized 10
80322 µ-Slide chemotaxis, Collagen IV: #1.5 polymer coverslip, sterilized 10