How to Run Wound Healing Assays to Get Maximum Biological Relevance and Reproducibility
Are you interested in...
- Creating the ideal workflow for your wound healing experiments?
- An effortless way to achieve consistent cell-free gaps?
- Biologically relevant real-time data on every single cell's behavior?
If so, we cordially invite you to participate in our next webinar, in cooperation with PHI.
The study of cell migration and cell motility is essential for understanding diseases. Cell migration can be the cause of cancer progression and metastatic cancer cells. Therefore, the wound healing assay is an established in vitro method for measuring directional cell migration.
However, measuring the kinematics of wound healing requires a cell-free gap between two cell-covered areas. The scratch assay—a typical way to create cell-free areas—shows poor reproducibility and user-dependent effects.
Having a reproducible experimental setup is the basis for achieving meaningful results.
Secondly, the results of conventional approaches are not showing the whole story. For example, end-point assays miss out on real-time data. Time-lapse setups overlook addressing the influence of cell migration and cell proliferation, or cannot quantify unique single-cell behavior. In addition, imaging using cellular markers is known to influence living cell behavior.
In this joint webinar, we will speak about the idea behind wound healing and migration assays. We will walk through a complete wound healing experimental workflow, from sample preparation and live cell imaging to quantitative image analysis, and then present you with multiple application examples, videos, and the latest user publications.
Dr. Peggy Benisch, Head of Sales, ibidi GmbH, Germany
Dr. Peggy Benisch studied Biology in Bayreuth, Würzburg, and Umeå, Sweden. She received her PhD in stem cell biology at the Orthopedic Center for Musculoskeletal Research in Würzburg, Germany. In 2013, she joined ibidi as an Application Specialist for the technical support of our customers worldwide. In 2017, she became the Head of the ibidi Academy, a program for training and conducting practical courses for ibidi partners and users. Since 2020, she has also been the Head of Sales at ibidi.
Lisa Bodily, Communication Manager, PHI, Sweden
Lisa attended Lund University, Sweden, where she received a degree in Molecular Biology (with a specialization in Molecular Genetics and Biotechnology). She joined PHI in 2019 as a HoloMonitor® Application Specialist, supporting researchers and users to help them obtain cell imaging and analysis results. Since 2021, she has been using her scientific expertise and hands-on lab experience as PHI's Digital Marketing and Communication Manager.