There will be a special focus on new applications that use the ibidi Pump System, such as impedance measurements, pulsatile flow, and the addition of substances to the flow system. The effect of shear stress parameters on cells will be elucidated by new internal experimental results, as well as by other publications.
An overview of various perfusion applications and experimental setups. The webinar will demonstrate the setup of a long term experiment, i.e., one lasting over several days, and provide microscopic pictures of endothelial cells. It will also highlight important experimental details and provide advice on how to troubleshoot through possible obstacles.
How you can culture endothelial cells under defined shear stress values? What is the basic physical background of laminar flow profiles and shear stress?
What equipment is required for a cell culture perfusion setup? Join ibidi webinar to find out the answers to these questions.
This webinar gives an overview of the detailed and defined analysis of the migration behavior of various cell types, such as endothelial cells, fibroblasts, cancer cells, and immune cells.
You will see a complete experiment with measured data using endothelial cells on a Matrigel® gel matrix. We will highlight important experimental details, plus give advice on output parameters and assay reproducibility.
One of the most prominent methods for studying directional cell migration in vitro. Measuring the kinematics of wound healing through the migration of cells requires a cell-free gap between two cell-covered areas. The scratch assay—a typical method for producing cell-free areas—shows poor reproducibility and user-dependent effects.
Do you want to know how a high throughput assay can be easily imaged and analyzed on a single cell level (e.g., for a T cell killing assay)? Have you ever wondered how multi cell arrays can be established with customized pattern sizes and shapes? Or do you want to learn how 3D cell aggregates (e.g., spheroids or organoids) can be immobilized on a slide, and thus imaged for days to even weeks?