René Remie Surgical Skills Centre

Microsurgical techniques course, module A - basic

Code PrMA

This 5-days training module can be of interest to clinical surgeons who want to master the basics of microsurgery in rodents. 

Training can be of interest to clinical surgeons who want to master the basics of microsurgery. It will typically involve:

  • blood vessel anastomoses (ETE, ETS, interposition grafts etc.)
  • nerve repairs
  • skin flap preparation
  • other techniques of particular interest to participants by agreement before or during the module.

Trainings are provided at Almere in The Netherlands. Ask us about training dates!

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Features

Day 1: Basic microsurgical techniques

  • 09.00 - 09.30 Welcome, introduction and expectations for the course
  • 09.30 - 10.45 Good Surgical Practice (GSP) in the rat
  • 10.45 - 11.00 Coffee and tea
  • 11.00 - 11.45 Instruments
  • 11.45 - 13.00 Suture materials and suture techniques

Practical exercises:

  • 14.00 - 15.30 Microsurgical suture techniques on a latex glove
  • 15.30 - 17.00 Anastomoses techniques on artificial vessels, using the MD PVC-Rat

Day 2: Experimental microsurgical techniques I

Practical exercises:

  • 09.00 - 10.15 Anastomoses techniques end-to-end (ETE) and end-to-side (ETS), using the MD PVC-Rat
  • 10.15 - 10.30 Coffee and tea
  • 10.30 - 12.30 Anastomosis techniques (ETS), portacaval shunt, using the MD PVC-Rat
  • 13.30 - 14.00 ETE anastomosis of the rat femoral artery (theory)

Practical exercises:

  • 14.00 - 17.00 Dissection techniques and vascular ETE anastomosis of the rat femoral artery

Day 3: Experimental microsurgical techniques II

  • 09.00 - 10.15 Anaesthesia including peri-operative care
  • 10.15 - 10.30 Coffee and tea

Practical exercises:

  • 10.30 - 17.00 ETE anastomosis of the femoral artery. ETE anastomosis of the jugular vein

Day 4: Experimental microsurgical techniques III

  • 09.00 - 09.45 Asepsis
  • 09.45 - 10.00 Coffee and tea

Practical exercises:

  • 10.00 - 17.00: Interposition abdominal aorta, carotid artery and femoral vein, nerve repair

Day 5: Experimental microsurgical techniques IV

  • 09.00 - 10.00 Discussion of participants special needs and requests
  • 10.00 - 10.15 Coffee and tea
  • 10.15 - 11.00 Discussion on technical approaches

Practical exercises:

  • 11.00 - 16.00 Requested techniques
  • 16.00 - 16.15 Course evaluation

Organizational information

Each participant has an own set-up with operating microscope, all (micro)surgical tools, suture materials and pathogen free laboratory rats and mice required for the course. The number of attendees per course is limited to 8 participants.

The registration fee includes the following items:

  • A numbered certificate
  • Hand-out of all PowerPoint slides used
  • Documentation on the surgical tools, catheters and operating microscopes used
  • Lunches, coffee, tea and soft drink