Tissue slice recovery chamber

• Six slice holding chambers
• Integrated gas bubbler
• Condensate flow eliminates the risk of osmotic shock
• Bath Thermistor for fast feedback
• Thermistor calibrated to ± 2° C
• Proportional Integral & Derivative Control algorithm
• Overheat Alarm

The Model 7470 Temperature-controlled Slice Holding chamber, has been developed to accompany the Campden Instruments range of tissue slicers.

The 7470 has been developed according to the principles described in the Plymouth Microelectrode Handbook (Gibb, A.J. and Edwards, F.A., 1994. Patch clamp recording from cells in sliced tissues. In Microelectrode Techniques: The Plymouth Workshop Handbook). The chamber was designed to hold and maintain tissue slices in six sub-chambers at physiological temperatures with an aeration supply to allow recovery from slicing as well as incubation during the experimental day (10-12 hrs).

The Proportional Integral & Derivative (PID) controlled heating element is integrated into the stainless steel bath bottom plate. All metal components are manufactured from high-grade stainless steel to remove the risk of contamination of the slice. Temperature feedback is via a thermistor probe attached to the bath wall at the same water as the specimen holder rings. We have also designed the chamber to reduce the risk of thermal/osmotic shock by ensuring the condensation on the lid does not drip back down on to the slice. The aeration pathway has been arranged so that the slice is not disturbed by the bubbles and the created fluid current helps retain the slices within the sub-chamber.