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Campden Vibrotom 700 smz-2
Campden Vibrotom 700 smz-2
Campden Instruments

Vibrotome SMZ

Code 7000smz-2Campden

Campden's top of the range high precision, vibrating microtome (vibrotome for short), this is the finest tissue slicer in the world for preparations for visual patch clamping or high resolution imaging. The 7000smz series Vibrating microtome has been the instrument of choice for many labs for 10 years - over 450 labs and counting!

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Neuroscience Laboratory Electrophysiology Isolated tissues and organs Pathomorphology
Features

Key features

  • Includes a z-axis calibration unit
  • Z-axis blade adjust minimizer
  • Blade holder angle to user requirement
  • Set start and stop position from blade travel.
  • Vibration speeds from 50 to 120 Hz
  • Amplitudes from 0.5mm to 2.5mm
  • Controlled blade advance at 10microns per sec
  • Manual or automatic operation
  • "Auto" programming by storage of the first slicing
  • Ice water bath easily removed for cleaning
  • Optional LED light and scope for clear observation

Using Campden microtomes, research detailing sectioning for visual patching of neurological tissue, heart, lung, and tissue scaffolds have all been published. The 7000smz-2 microtome represents significant advances with higher precision at a lower cost.

The vibrating mechanism of the 7000smz-2 microtome delivers unprecedented accuracy with nearly silent operation. Every machine is tested before dispatch by our in-house measurement devices and each machine is supplied with its own z-axis calibration checker.

The effect of excessive Z-axis deflection on the health and viability of the tissue preparation has been much discussed since the publication of Geiger et al (2003) and the Campden 7000smz-2 tissue slicer, with ≤ 1µm Z-axis deflection across a wide range of vibration speeds and amplitudes, delivers healthy viable sections every time.

The user interface is easy and versatile (with no foot switch unlike previous models) and offers simple operation at the push of a button or a range of changeable parameters for the expert user. The Campden 7000smz-2 also provides longevity of performance, as not only does the slicer give ≤ 1 µm performance out of the box, but the advance vibrating mechanism does not contain bearings and other elements subject to wear. Thus it will retain the ≤ 1 µm z-ad performance for years to come, giving you consistency in your biological preparation, without the need for expensive servicing.

Holding the tissue close to 4° C must continue throughout the slicing procedure. This can be done with passive cooling where a known amount of ice is used to maintain the cooled a.c.s.f. or with an electronically controlled thermo-electric Tissue Bath Cooler (7610A).

Campden developed both ceramic and stainless steel microtome blades, alongside the machine, so that the grinding and honing facets for the blade edge are matched to the blade downward angle of orientation to the tissue. The ceramic blades have a particular advantage when slicing brain tissue older than 30 days. These ceramic microtome blades also have a second advantage in that unlike stainless blades, these do not have to be replaced after each tissue sample.

Other options include an LED cold light and magnifier or scope for clear observation whilst slicing. Shown with optional Slice incubation chamber and temperature controller.

Specifications
  • Section thickness step size: 0.001 mm
  • Bath table rise & fall speed: 1.0 mm/sec maximum
  • Maximum (vertical) travel of bath table: 19 mm
  • Cutting head advance speed:
    • Minimum: -4.0 mm/sec (-1.00 during slicing)
    • Maximum: +4.0 mm/sec (+1.00 during slicing)
  • Cutting head retraction speed: 4.0 mm/sec
  • Maximum travel of cutting head: 40 mm
  • Blade oscillation frequency:
    • Minimum: 50 Hz (lower frequenices available upon request)
    • Maximum: 120 Hz (amplitude dependent)
  • Frequency step size: 5 Hz
  • Blade oscillation amplitude
    • Minimum: 0.5 mm (nominal)
    • Maximum: 2.5 mm (nominal)
  • Amplitude step size: 0.25 mm (nominal)
  • Power requirements (Selectable): 115VAC 60Hz, 230VAC 50Hz
  • Power rating: 100W
  • Fuse rating
    • 115V: T2A 250VAC
    • 230V: T2A 250VAC
  • Light source: 100-240Vac 3W
  • Dimensions: 410mm Width x 400mm Depth x 270mm Height (excluding light source and microscope)
  • Weight: 33 kg (excluding microscope)
  • Boxed shipping weight: 60 kg (excluding microscope)

 

Components
Extend system with following add-ons
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Comparison of Campden Vibrating Microtomes
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Generation of precision cut-lung slices (PCLS) for ex vivo modeling of lung disease and drug testing
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Preparation of high quality rodent hippocampal brain slices for experimental studies of synaptic plasticity and epileptiform activity
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VibroTome bibliography - references
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Publications

  • Preparation of viable adult ventricular myocardial slices from large and small mammals
    Samuel A Watson, Martina Scigliano, Ifigeneia Bardi, Raimondo Ascione, Cesare M Terracciano & Filippo Perbellini

    Nature Protocols volume 12, pages 2623-2639 (2017) - Zobacz abstrakt

  • Free-of-Acrylamide SDS-based Tissue Clearing (FASTClear) for three dimensional visualization of myocardial tissue
    Filippo Perbellini, Alan K. L. Liu, Samuel A. Watson, Ifigeneia Bardi, Stephen M. Rothery & Cesare M. Terracciano

    Scientific Reports 7, Article number: 5188 (2017) - Zobacz abstrakt

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