RWD Life Science

R811 Dual color multichannel fiber photometry system

Code R811

R811 dual color multichannel fiber photometry system is compatible with optoelectronics equipment. It has different wavelengths of excited light, 410nm and 470nm, of which 410 is used to acquire reference signal and eliminate noise. The system can record signal of green fluorescence indicator like GCaMP and dLight or neurotransmitter probe.
And also it can be applied to record the neural signal for 9 sites at the same time.

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Features

Overview

The fiber photometry system records changes in the fluorescence intensity of neurons in a specific brain area to reflect neuronal population activity. In the study of neural circuits, the fiber photometry system can perform long-term stable monitoring of the neurons of freely moving animals, and explore the correlation between neural activity and animal behavior.

  • Main Applications
    Ca2+ and neurotransmitter signal detection
    Study the function of neural circuits
    Explore the mechanisms of neurological diseases
    Develop new fluorescent sensor probe
    Experiment of optical principle
  • Advantages
    Two kinds of excitation light sources, that is 410 nm and 470 nm, are respectively used for excitation of reference and green fluorescence ;
    Support up to 9 channels, suitable for simultaneous experiment of multiple animals or multiple brain locations;
    Professional acquisition and analysis softwares are flexible and easy to operate with data processing functions available. No matlab programming is required;
    Supports multiple acquisition modes including continuous acquisition, interval acquisition, acquisition upon trigger, delayed acquisition and timing acquisition;
    Live display of DeltaF/F acquisition to check scale of signal changes during acquisition;
    Customized adjustment of output signal parameter, easily trigger and control external excitation equipment to achieve closed-cycle control of excitation and recording;
    Compatible with optogenetics for recording and stimulation at the same site.
  • Professional Acquisition and Analysis Softwares
    Professional acquisition and analysis software enable stable data acquisition and easy processing. Data analysis
    includes data clipping, bleaching correction, smoothing, movement correction, event heat map, peak statistics,
    area under curve and heat map of behavior trajectory.
    Generation of Peri-event heat map with one click. Freely choose and handle events of interest, and flexibly add or remove events. The results can be easily saved and exported to DetaF/F, Z-score, Peri-event, peak statistics and AUC. Information in the image can be freely edited by saving the image as editable SVG format.
Diagram.png
Stable Data Acquisition
heatmap.png
Fluorescence Comparison
Specifications
  • Wavelength of excitation light: 410nm 470nm
  • Power: Min 0 µW, Max ≥ 100 µW, adjustable with an accuracy of 0.1 µW
  • Number of channels: 9
  • Frame rate of fluorescent sampling: Max 250fps
  • Digital signal interface: 4 Input and 4 Output
  • Signal output: Output frequency 0-500Hz, adjustable output pulse width and duration
  • Marking: Manual marking (10), automatic marking (4), ROI marking (9)
  • Behavior camera: 1920*1080(30fps) 1280*720(60fps) . Switchable among multiple frame rates of resolution
  • Compatible: Compatible with optogenetics for recording and stimulation at the same site