NucleoCounter® NC-3000™
Advanced image cytometer
- A variety of fixed and build-your-own assays
- High-speed cell counting and advanced data analysis
- No warm-up or calibration required
- No liquid waste produced
- 21 CFR Part 11/GMP-ready software
The NucleoCounter® NC-3000™ advanced image cytometer utilizes fluorescence imaging to characterize cell properties. The instrument performs high-speed plug-and-play analytical assays of mammalian, yeast and insect cells. Pre-defined assays include a unique 5-minute cell cycle assay, a selection of apoptosis assays and the standard cell count and viability assay.
With the FlexiCyte™ license, you can create protocols with options for light source, emission filters and other detailed settings. The accompanying NucleoView™ software is ideal for intuitive data analysis and has unlimited licenses. You can view data in plot graphs and interlinked images, which can be saved for later on-demand analysis.
This advanced image cytometer ensures flow-like analysis with high reproducibility, operates with low sample volumes, and is calibration-free.
Used with the disposable Via1-Cassette™, this closed cell counting system helps you stay protected when handling samples. No pipettes, no exposure to (toxic) reagents, and minimal clean-up make the NucleoCounter® NC-3000™ a safe option.
Read more about the Cassette technology
- Assays: 5-minute cell cycle analysis, 1-minute apoptosis assay, 1-minute GFP assay
- Exports to data analysis tools, such as FCS Express and FlowJo
- PDF reports
- Unlimited software installs and free updates
- 5-channel open platform
- Application type(s): Fixed assays: Cell count & viability, Cell cycle, Mitochondrial potential, Vitality, Caspase, Annexin V, Transfection efficiency, and DNA fragmentation assays. User-adapted protocols with the Flexicyte™ module
- Cell type(s): Mammalian, yeast, insect, avian
- Software: NucleoView™
- Consumable(s): Via1-Cassette™, NC-Slide A2™ and NC-Slide A8™
- Capacity (Analysis time): Variable, depending on consumable and assay type
- Analysis volume: 3.2 μl sample (Via1-Cassette™), 0.8 – 16 μl sample (NC-Slide A2™, NC-Slide A8™)
- Optimal range: 5×104 to 5×106 cells/ml (for counting)
- Staining dye(s): Hoechst 33342, JC-1, VitaBright-43™, VitaBright-48™, Acridine orange (AO), DAPI, Propidium iodide (PI), User-defined
- Loading volume: 60 μl (Via1-Cassette™), 30 μl (NC-Slide A2™), 10 μl (NC-Slide A8™)
- Excitation: Seven LED light sources with peaks at 365 nm, 405 nm, 475 nm, 530 nm, 630 nm + 2 white LEDs
- Emission: Nine interchangeable emission filters: 430/20, 470/55, 475/15, 530/15, 560/35, 580/25, 675/75, 630 LP, 740/60
- Optics: Lens with x2 magnification, 1/2″ CCD camera with 1392×1040 pixels
- Data output: Images, tables, histograms, scatter plots
- Data export formats: .cm, .csv, .pdf, .cmpp, .bmp, .tiff, .acs, .fcs
- Product number: 970-3002 (Fixed Analytical Assays); 970-3003 (FlexiCyte™)
- Size: 29 x 29 x 31 cm (W x H x D)
- Weight: 14 kg (30.9 lb)
- Supply voltage: 24VDC (100–240 V ~ 50–60 Hz)
