ChemoMetec

NucleoCounter® NC-3000™

Advanced image cytometer

  • A variety of fixed and build-your-own assays
  • High-speed cell counting and advanced data analysis
  • No warm-up or calibration required
  • No liquid waste produced
  • 21 CFR Part 11/GMP-ready software
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Features

The NucleoCounter® NC-3000™ advanced image cytometer utilizes fluorescence imaging to characterize cell properties. The instrument performs high-speed plug-and-play analytical assays of mammalian, yeast and insect cells. Pre-defined assays include a unique 5-minute cell cycle assay, a selection of apoptosis assays and the standard cell count and viability assay.

With the FlexiCyte™ license, you can create protocols with options for light source, emission filters and other detailed settings. The accompanying NucleoView software is ideal for intuitive data analysis and has unlimited licenses. You can view data in plot graphs and interlinked images, which can be saved for later on-demand analysis.

This advanced image cytometer ensures flow-like analysis with high reproducibility, operates with low sample volumes, and is calibration-free.

Used with the disposable Via1-Cassette™, this closed cell counting system helps you stay protected when handling samples. No pipettes, no exposure to (toxic) reagents, and minimal clean-up make the NucleoCounter® NC-3000™ a safe option.

Read more about the Cassette technology

  • Assays: 5-minute cell cycle analysis, 1-minute apoptosis assay, 1-minute GFP assay
  • Exports to data analysis tools, such as FCS Express and FlowJo
  • PDF reports
  • Unlimited software installs and free updates
  • 5-channel open platform
Specifications
  • Application type(s): Fixed assays: Cell count & viability, Cell cycle, Mitochondrial potential, Vitality, Caspase, Annexin V, Transfection efficiency, and DNA fragmentation assays. User-adapted protocols with the Flexicyte™ module
  • Cell type(s): Mammalian, yeast, insect, avian
  • Software: NucleoView™
  • Consumable(s): Via1-Cassette™, NC-Slide A2™ and NC-Slide A8™
  • Capacity (Analysis time): Variable, depending on consumable and assay type
  • Analysis volume: 3.2 μl sample (Via1-Cassette™), 0.8 – 16 μl sample (NC-Slide A2™, NC-Slide A8™)
  • Optimal range: 5×104 to 5×106 cells/ml (for counting)
  • Staining dye(s): Hoechst 33342, JC-1, VitaBright-43™, VitaBright-48™, Acridine orange (AO), DAPI, Propidium iodide (PI), User-defined
  • Loading volume: 60 μl (Via1-Cassette™), 30 μl (NC-Slide A2™), 10 μl (NC-Slide A8™)
  • Excitation: Seven LED light sources with peaks at 365 nm, 405 nm, 475 nm, 530 nm, 630 nm + 2 white LEDs
  • Emission: Nine interchangeable emission filters: 430/20, 470/55, 475/15, 530/15, 560/35, 580/25, 675/75, 630 LP, 740/60
  • Optics: Lens with x2 magnification, 1/2″ CCD camera with 1392×1040 pixels
  • Data output: Images, tables, histograms, scatter plots
  • Data export formats: .cm, .csv, .pdf, .cmpp, .bmp, .tiff, .acs, .fcs
  • Product number: 970-3002 (Fixed Analytical Assays); 970-3003 (FlexiCyte™)
  • Size: 29 x 29 x 31 cm (W x H x D)
  • Weight: 14 kg (30.9 lb)
  • Supply voltage: 24VDC (100–240 V ~ 50–60 Hz)