SCIENTZ

Benchtop Gene Gun

Code GJ-1000
Bench-top, gas-driven biolistic particle delivery system that uses a rupturable-membrane shock wave (3–12 MPa) to bombard cells, tissues, organs, microbes and small whole plants with gold or tungsten DNA-, RNA- or protein-coated microparticles for efficient laboratory gene transfer.
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Features

GJ-1000 High Pressure Gene Gun

The GJ-1000 is a bench-top, gas-driven biolistic particle delivery system (gene gun) for laboratory-scale gene transfer. A rupturable membrane, made of polyimide film in different thicknesses, regulates the helium pressure inside the chamber. When the pressure reaches the membrane’s critical point, it bursts and releases a powerful shock wave that drives the particle carrier downward at high speed to bombard the target cells, tissues or organs. Working pressure is adjustable between 3 and 12 MPa, allowing the system to be optimised for a wide range of sample types.

Features

  • High conversion rate – biologically inert particle carriers improve safety and precision
  • High versatility – gas-driven design simplifies operation and widens the range of applications
  • Optional carrier membranes – flying or steel membranes available depending on the receptor
  • Adjustable pressure – optimised between 3–12 MPa for different sample types
  • Quick cleaning – detachable circular chamber allows easy sterilisation

Technical Parameters

  • Target area: up to 40 cm²
  • Working pressure range: 3–12 MPa
  • Target type: animal cell and organ cultures; small whole plants, cultured plant cells and exosomes; yeast, bacteria and other microbial cells; chloroplasts and mitochondria
  • Voltage: 220 V / 50 Hz
  • Gas source: helium or nitrogen (helium recommended for optimal results)
  • Metal particles: Φ0.8–1.5 µm / Φ1.5–3.0 µm gold or tungsten powder

Standard System Configuration

Main unit, vacuum pump, and consumables for 1000 shots (3.5/4.5/7 MPa rupturable membranes, carrier membrane, stainless steel mesh, 2 bottles of gold powder, 1 bottle of tungsten powder).

Applications of GJ-1000 High Pressure Gene Gun

  • Research on transient manifestations of gene function
  • Studies of functional stability
  • Genetic and cancer-related research
  • DNA vaccine research and development
  • Gene therapy research
  • Transgenic plant research
  • Genetic engineering and drug-related research

Typical fields of use include the food industry, agricultural and forestry sciences, biological engineering, and animal medicine.

Specifications
  • Target area: up to 40 cm²
  • Working pressure range: 3-12 MPa
  • Target type: animal cell/organ cultures, small whole plants, cultured plant cells, exosomes, yeast, bacteria, other microbial cells, chloroplasts, mitochondria
  • Voltage: 220V/50Hz
  • Gas source: helium or nitrogen (helium optimised)
  • Metal particles: Φ0.8-1.5 µm / Φ1.5-3.0 µm gold or tungsten powder
Publications
1. YE S, JIANG Y, DUAN Y, et al. Constitutive expression of the poplar WRKY transcription factor PtoWRKY60 enhances resistance to Dothiorella gregaria Sacc. in transgenic plants. Tree Physiology, 2014. 2. Jianan Li, Jun Zou, et al. Plasmid DNA could be delivered into Eimeria maxima unsporulated oocyst with gene gun system. Acta Veterinaria Hungarica, 2012. 3. Liang Sun, Shutong Liu, et al. Optimization of particle bombardment conditions by β-glucuronidase (GUS) reporter system in tomato fruit. African Journal of Biotechnology, 2011.