Sutter Instrument
P-2000 Laser-Based Micropipette Puller
Code P-2000
The P-2000 is Sutter Instrument's CO2 laser-based micropipette puller — the only puller capable of working with quartz (fused silica) glass. Available in two configurations (P-2000/G for OD ≥ 0.6 mm; P-2000/F for OD < 0.6 mm), it produces micropipettes, patch pipettes, optic fibers, and electrospray/nanospray tips with unmatched precision.
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Features
P-2000 — The Gold Standard for Quartz Glass Micropipette Pulling
The P-2000 Laser-Based Micropipette Puller from Sutter Instrument is the only micropipette puller capable of working with quartz (fused silica) glass — the strongest and most chemically pure glass available for scientific applications. Using a precisely modulated and directed CO2 laser as its heat source, the P-2000 delivers unparalleled control over pipette taper geometry across an exceptional range of glass diameters from 0.125 mm to 1.8 mm (model dependent).
Two Models — One for Every Application
- P-2000/G — optimized for glass OD ≥ 0.6 mm (up to 1.5 mm quartz / 1.8 mm conventional); ideal for patch pipettes, intracellular electrodes, and microperfusion needles
- P-2000/F — optimized for glass/fiber OD < 0.6 mm (down to 0.125 mm); designed for optical fiber tapering, nano-electrospray ionization emitters from fused silica capillary tubing, and near-field scanning microscopy probes
Key Features
- CO2 laser heat source — enables pulling of quartz glass (impossible with filament pullers)
- Microprocessor-controlled pull cycle with up to 100 stored programs, each with up to 8 command lines
- 5 independently adjustable parameters per cycle line: HEAT, FILAMENT, VELOCITY, DELAY, PULL — providing exquisite control of taper geometry
- Produces 2 identical pipettes per pull; tip diameters 0.03–5 µm (glass) or 0.01–5 µm (fiber)
- Maximum taper length: 1.8 cm; maximum glass OD: 1.65 mm quartz / 1.8 mm borosilicate
- Write-protection for programs; Ramp Test function to establish optimal heat settings
- Copy program function for rapid duplication of pulling protocols
- Class I laser product (safe to operate as shipped; 20W CO2 laser with full safety enclosure and interlocks)
- Dual voltage/frequency: 100–120 VAC / 200–240 VAC, 50–60 Hz
Applications of P-2000 System
- Patch clamp pipette fabrication from quartz glass
- Intracellular recording electrodes
- Microinjection and microperfusion needles
- Optical fiber tapering and near-field scanning probe fabrication
- Electrospray and nanospray ionization emitters (HPLC, mass spectrometry)
Specifications
- Heat source: CO2 laser (20W Class IV, enclosed — Class I product as shipped)
- Glass type: Quartz (fused silica) and conventional glasses (borosilicate, aluminosilicate)
- Models: P-2000/G (OD ≥ 0.6 mm) / P-2000/F (OD < 0.6 mm)
- Glass OD range: 0.125–1.2 mm (optimal); up to 1.65 mm quartz / 1.8 mm borosilicate (P-2000/G)
- Tip size: 0.03–5 µm (glass); 0.01–5 µm (fiber)
- Max taper length: 1.8 cm
- Programs: 100 programs × 8 command lines each
- Cycle parameters: HEAT, FILAMENT, VELOCITY, DELAY, PULL (5 per line)
- Usable pipettes / pull: 2
- Cooling: Laser on/off (no compressed air required)
- Electrical: 100–120 VAC / 200–240 VAC, 50–60 Hz; max 396 VA
- Dimensions (H×W×D): 33 × 76 × 37 cm (13 × 30 × 14.5 in)
- Weight: 36.4 kg (80 lb)
Components
Standard system components
- P-2000 P-2000/G Laser-Based Micropipette Puller (OD ≥ 0.6 mm)
- P-2000/F P-2000/F Laser-Based Micropipette Puller (OD < 0.6 mm, optical fibers)