R821 Tricolor Multichannel Fiber Photometry System

Overview

The fiber photometry system records changes in the fluorescence intensity of neurons in a specific brain area to reflect neuronal population activity. In the study of neural circuits, the fiber photometry system can perform long-term stable monitoring of the neurons of freely moving animals, and explore the correlation between neural activity and animal behavior.

• Main Applications:
  Ca2 + and neurotransmitter signal detection
  Study the function of neural circuits
  Explore the mechanisms of neurological diseases
  Develop new fluorescent sensor probe
  Experiment of optical principle
• Advantages
  Three kinds of excitation light sources, that is 410nm、470nm and 560nm, are respectively used for excitation
  of reference, green fluorescence and red fluorescence;
  Support up to 9 channels, suitable for simultaneous experiment of multiple animals or multiple brain
  locations;
  Dual highly sensitive detectors enabling independent and sequential detection to avoid interference of
  fluorescence excitation and detection, acquiring more accurate signal;
  Professional acquisition and analysis softwares are flexible and easy to operate with data processing functions
  available. No matlab programming is required;
  Supports multiple acquisition modes including continuous acquisition, interval acquisition, acquisition upon
  trigger, delayed acquisition and timing acquisition;
  Live display of DeltaF/F acquisition to check scale of signal changes during acquisition;
  Customized adjustment of output signal parameter, easily trigger and control external excitation equipment
  to achieve closed-cycle control of excitation and recording;
  Compatible with optogenetics for recording and stimulation at the same site.

• Successful Case
  Journal：Cell Stem Cell
  Demand of the Product：We recorded calcium dynamics in DG-projecting SuM neurons by fiber photometry. In vivo recordings were carried out in an
  open-top HC (21.6×17.8×12.7 cm3) or EE (45×25× 20 cm3) containing toys, colorful balls, domes or tunnels and food, for 10min.
  Cite Reference：Li, YD., Luo, YJ., Chen, ZK. et al. Hypothalamic modulation of adult hippocampal neurogenesis in mice confers activity-dependent regulation of memory and anxiety-like behavior. Nat Neurosci 25, 630–645 (2022). https://doi.org/10.1038/s41593-022-01065-x

Journal：Nature Neuroscience
Demand of the Product：We performed fiber photometry to record calcium activity of CA3 and CA1 pyramidal neurons labeled with GCaMP6f upon chemogenetic activation of SuM-enhanced ABNs by intraperitoneal CNO injection.
Cite Reference：Li YD, Luo YJ, Xie L, Tart DS, Sheehy RN, Zhang L, Coleman LG Jr, Chen X, Song J. Activation of hypothalamic-enhanced adult-born neurons restores cognitive and affective function in Alzheimer’s disease. Cell Stem Cell. 2023 Apr 6;30(4):415-432.e6. doi: 10.1016/j.stem.2023.02.006