Instech

Rat continuous infusion plus blood sampling

A two-channel system for continuous infusion on one channel and low-stress blood sampling on the second.

Catheterize two separate vessels and use a two-channel system to collect manual blood samples outside the cage without touching the animal, all while infusing continuously through a swivel.

The blood collection line uses PE/PVC tubing for minimal evaporation and terminates in a PinPort™ for simple, clean access with a syringe fitted with a PNP3M injector or a Sarstedt capillary collection tube with a PNP3MS connector (arterial pressure is required for capillary collection tubes).

If you are already performing surgery to place the IV dosing catheter, why not take the simple extra step of placing a blood sampling catheter to avoid the pain, stress and labor of peripheral bleeds? 

If your institute does not have a surgeon on site, in many parts of the world Charles River and Envigo can implant the catheters and two-channel Vascular Access Button™ as a surgical service so that the animals arrive at your lab recovered from surgery and ready for use.

You can take this system a step further by connecting the blood sampling line through a two-channel swivel to an automated blood sampler.

See below for a typical application setup with a list of components. If you need any assistance in selecting other setup parts, please don't hesitate to contact the Animalab team.

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Components
Standard system components
  • HA1100 Pump 11 Elite infuse-only single syringe pump (704500)
  • CM375KRP Snap-in spring swivel arm for rat shoebox cage top and plastic swivel
  • KVABR2T/25 2ch rat VAB tether kit, 12in (30cm) spring; red=CoEx, PinPort; white=PU tubing, 375/25PS, 24in CoEx, LS25
  • VABR2B/25R22 Vascular Access Button for rat, magnetic, 2 channel, 25ga red, 22ga white, injector
  • C30PU-RJV2326 Catheter for rat jug vein, PU 3Fr 20cm, 2.6cm insertion length (a2.4), rounded tip. 22ga luer stub.
  • C30PU-RCA1613 Catheter for rat carotid artery, PU 3Fr 20cm, collar @ 2.5cm. 22ga luer stub.
  • PNP3M PinPort injectors, 50 sterile pouches of 5 (250 pcs)
  • PNP3MS PinPort-to-Sarstedt capillary tube connector, 50 sterile pouches of 5 (250 pcs)
  • CT-CB300-K2EDTA Capillary / centrifugation tube, 300µL, K2EDTA, purple (Sarstedt Microvette CB 300 16.444.100)
  • USP-HS-020-1-NJ1P-50 Sterile catheter flush solution, heparin (20 USP units/mL) in 0.9% saline, 50 pcs (10 packs of 5) 1mL in 1mL syringe, PinPort injector
  • USP-S-1-BD1P-50 Sterile catheter flush solution, 0.9% saline, 50 pcs (10 packs of 5) 1mL in 1mL syringe, PinPort injector