DNA Shearing System with Auto-Tuning 20–400 kHz
The SCIENTZ08-IIIA is a non-contact ultrasonic DNA shearing system that shears, homogenizes and mixes samples by an isothermal, non-contact method. It enables sterile, ultra-micro disruption and shears chromosomes directly through the walls of sealed centrifuge tubes. Tailored to sample pretreatment for next-generation sequencing (NGS) and chromatin immunoprecipitation (ChIP), it offers high throughput, low sample loss and no cross-contamination for laboratories that process many or precious samples every day.
Its key feature is broadband ultrasound: the operating frequency adapts automatically within 20–400 kHz. The ultrasonic generator sits at the bottom of the water tank, so the whole bath receives wide, uniform energy with reduced foaming, and continuous automatic rotation of the tubes evens out the energy distribution further. A constant-temperature water bath is included as standard, keeping samples between 2 and 40 °C. The instrument is CE and RoHS certified.
Key Features
- Broadband ultrasound – frequency adapts automatically within 20–400 kHz
- Non-contact, closed-tube processing – no aerosols and no cross-contamination, enhanced biosafety
- High throughput – up to 60 × 0.1 ml samples per run
- Rotating sample holder – continuous automatic rotation for uniform energy distribution
- Isothermal processing – constant-temperature water bath included, 2–40 °C with 0.1 °C precision
- 7-inch touch screen and storage of 50 data sets
- Wide time range – from 0.1 s to nearly 200 hours
- Adjustable power – 1–100%
Working Principle
Unlike probe sonicators, which act only in a small area near the probe tip, the SCIENTZ08-IIIA places the ultrasonic generator at the bottom of the water tank. Ultrasonic energy passes through the tube walls into the sealed samples, where cavitation shears DNA and chromatin and disrupts cells without any contact between the transducer and the sample.
Applications of the SCIENTZ08-IIIA DNA Shearing System
- DNA shearing for next-generation sequencing (NGS)
- Chromatin immunoprecipitation (ChIP) sample preparation
- Cell disruption and protein extraction
- Inclusion body extraction
- Micro-emulsification
- Food, agricultural, forestry and bioengineering research
- Frequency: automatically adapts within 20–400 kHz
- Display: 7-inch high-precision touch screen
- Ultrasonic power: 2500 W
- Power adjustment: 1–100%
- Total ultrasonic time: 0.1 s to nearly 200 h
- Working time: 0.1–99.9 s
- Pause time: 0–99.9 s (0 = continuous mode)
- Duty cycle: 1–99.9%
- Water tank volume: 2.3 L
- Sample processing capacity: up to 60 × 0.1 ml
- Standard adapters: 2 sets 8 × 1.5/2 ml, 2 sets 20 × 0.5 ml, 2 sets 30 × 0.1 ml
- Data storage: 50 sets
- Cooling system: constant-temperature water bath (standard)
- Temperature range: 2–40 °C
- Temperature precision: 0.1 °C
- Voltage: 220 V / 50 Hz
- Certification: CE, RoHS
- Ultrasonic main unit with water tank
- Rotating sample assembly
- Constant-temperature water bath
- 2 sets of 8 × 1.5/2 ml racks
- 2 sets of 20 × 0.5 ml racks
- 2 sets of 30 × 0.1 ml racks
