DNA Shearing System with Auto-Tuning 40-100 kHz
The SCIENTZ08-IIIB is a non-contact ultrasonic DNA shearing system that shears and mixes samples by an isothermal, non-contact method. It enables sterile, ultra-trace fragmentation and shears chromosomes directly through the walls of sealed centrifuge tubes. Tailored to DNA sample pretreatment for next-generation sequencing (NGS) and chromatin immunoprecipitation (ChIP), it offers high throughput, low sample loss and no cross-contamination for laboratories that process many or precious samples every day.
The operating frequency adapts automatically within 40–100 kHz. Ultrasonic transducers mounted on the outside of the water tank place the entire tank within the ultrasonic field, ensuring wide, uniform energy distribution with reduced foaming, while continuous automatic rotation of the tubes evens out the energy distribution further. An independent constant-temperature unit is included as standard.
Key Features
- Auto-tuning ultrasound – frequency adapts automatically within 40–100 kHz
- Non-contact, closed-tube processing – no aerosols and no cross-contamination, enhanced biosafety
- High throughput – up to 60 × 0.1 ml samples per run; optional racks for 5, 10 and 50 ml tubes
- Rotating sample holder – continuous automatic rotation for uniform energy distribution
- Isothermal processing – independent constant-temperature unit included
- 7-inch touch screen and storage of up to 50 experimental data sets
- Wide time range – from 0.1 s to 199 h 59 min 59.9 s
- Adjustable power – 1–100%
Working Principle
In probe sonicators, microflow and cavitation occur only close to the probe, giving high intensity over a small area. In the SCIENTZ08-IIIB the transducers are mounted outside the water tank, so the whole tank lies within the ultrasonic field. Energy passes through the tube walls into the sealed samples, where cavitation shears DNA and chromatin without any contact between the transducer and the sample.
Applications of the SCIENTZ08-IIIB DNA Shearing System
- DNA fragmentation for next-generation sequencing (NGS)
- Chromatin immunoprecipitation (ChIP) sample preparation
- Animal cell disruption
- Protein and inclusion body extraction
- Micro-emulsification
- Food, agricultural, forestry and bioengineering research
- Frequency: 40–100 kHz, automatically adaptive
- Display: 7-inch high-precision touch screen
- Ultrasonic power: 2500 W (0–100%)
- Total ultrasonic time: 0.1 s–199 h 59 min 59.9 s
- Working time: 0.1–99.9 s
- Interval time: 0–99.9 s (0 = continuous mode)
- Duty cycle: 0.1–99.9%
- Water tank capacity: 2.3 L
- Sample processing capacity: max. 60 × 0.1 ml
- Standard adapters: 2 sets 8 × 1.5/2.0 ml, 2 sets 20 × 0.5/0.65 ml, 2 sets 30 × 0.1/0.2 ml
- Optional adapters: 5 × 5 ml, 6 × 10 ml, 3 × 50 ml
- Data storage: 50 sets
- Cooling system: independent constant-temperature unit (standard)
- Voltage: 220 V / 50 Hz
- Ultrasonic main unit with water tank
- Rotating sample assembly
- Constant-temperature unit
- 2 sets of 8 × 1.5/2.0 ml racks
- 2 sets of 20 × 0.5/0.65 ml racks
- 2 sets of 30 × 0.1/0.2 ml racks
