Gene Electroporator Advanced

GP-3000 Gene Electroporator

The GP-3000 Gene Electroporator consists of the main unit, a gene electroporation cup and dedicated connecting cables. It uses electroporation to introduce DNA into competent cells, plant and animal cells, and yeast cells. Compared with other transformation methods, it offers high repeatability, high efficiency, ease of operation and precise control, and because electroporation is a physical rather than a chemical or viral process, it causes negligible genotoxicity. Compared with the SCIENTZ-2CS, the GP-3000 adds a square-wave pulse mode alongside exponential decay, finer-grained low- and high-voltage capacitance adjustment, and configurable multi-pulse discharge for more demanding protocols.

Working Principle

Electroporation (cellular electroporation) introduces exogenous macromolecules – DNA, RNA, siRNA and proteins, as well as small molecules – into cells. Under a strong, momentary electric field, the cell membrane becomes permeable, allowing charged exogenous substances to cross it in a manner similar to electrophoresis. Because the phospholipid bilayer has high resistance, most of the applied voltage is borne by the membrane, with minimal current reaching the cytoplasm and very low voltage at the nuclear membrane, keeping cytotoxicity and genotoxicity negligible. As a physical method, electroporation is largely unaffected by the molecular properties of the cell surface, so it can be applied to virtually any cell type and its results are easy to quantify.

Features

• High efficiency – short transformation time, high conversion rate, excellent repeatability
• Intelligent storage – experimental parameters can be stored and easily retrieved
• Precise control – microprocessor-controlled pulse discharge
• Aesthetic, integrated design – intuitive touchscreen display, easy operation

Technical Parameters

• Pulse type: exponential decay and square wave
• High voltage output: 401–3000 V
• Low voltage output: 50–400 V
• Power supply: 100–240 VAC, 50/60 Hz, 100 W
• Number of continuous discharges: 1–9
• Low voltage capacitance: 25–1575 µF, in 1 µF increments (25 µF increments recommended)
• High voltage capacitance: 10–60 µF, in 1 µF increments (10/25/35/50/60 µF recommended)
• Parallel resistance: 50–1650 Ω, in 50 Ω steps
• Discharge and interval time: 0.1–999 ms, in 0.1 ms increments
• Control system: microcomputer-controlled, with adjustable RC time constant
• Main unit dimensions: 30 × 20 × 20 cm; net weight 4.5 kg

Standard System Configuration

Main unit, gene electroporation cup, dedicated connecting cables.

Indicative Electroporation Parameters by Organism

Note: parameters vary between labs and are provided for reference only.

Applications of GP-3000 Gene Electroporator

• Electroporation of bacteria, yeast and other microorganisms
• Transfection of mammalian cells, plant tissues and protoplasts
• Cell hybridisation and gene fusion
• Introduction of marker genes for labelling and indicating purposes
• Introduction of drugs, proteins, antibodies and other molecules for studying cellular structure and function

Typical fields of use include the food industry, agricultural sciences, veterinary research and bioengineering.