Cell Culture Cup Chambers for TEER Measurement – EndOhm

Cell Culture Cup Chambers for TEER Measurement – EndOhm

Urządzenie do pomiaru TEER nabłonkowych i śródbłonkowych hodowli komórkowych - Kompatybilne z EVOM2. Wykorzystując miernik rezystancji EVOM2 ™ WPI, komory Endohm zapewniają odtwarzalne pomiary rezystancji pojedynczych warstw śródbłonkowych i nabłonkowych w insertach hodowlanych. 

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Cell Culture Cup Chambers for TEER Measurement – EndOhm

For TEER measurement of epithelial and endothelial cell cultures

  • Compatible with EVOM2
  • Improved accuracy of 1-2 Ohm
  • Sterilize with EtO, alcohol, or a bactericide
  • Choose Culture Cup: 12mm (ENDOHM-12), 24mm (ENDOHM-24SNAP) or 6mm (ENDOHM-6)

Benefits

  • Stability and reproducibility superior to the STX2 electrodes
  • Can be used with 6, 12 or 24 well plates with removable wells
  • Symmetrical electrode pattern disperses test current uniformly
  • Simple test procedure to verify electrode performance

Applications

  • TEER measurement for removable culture cup systems using

TEER measurement in individual cups

Using WPI’s EVOM2™ resistance meter, Endohm chambers provide reproducible resistance measurements of endothelial and epithelial monolayers in culture cups. Transfer cups from their culture wells to the Endohm chamber for measurement rather than using hand-held electrodes. The chamber and the cap each contain a pair of concentric electrodes: a voltage-sensing silver/silver chloride pellet in the center plus an annular current electrode. The height of the top electrode can be adjusted to fit cell culture cups of different manufacture.

Make more precise measurements with Endohms

Endohm’s symmetrically opposing circular disc electrodes, situated above and beneath the membrane, allow a more uniform current density to flow across the membrane than with STX2 electrodes. The background resistance of a blank insert is reduced from 150 Ω (when using WPI’s hand-held STX2 electrodes) to less than 5 Ω. With Endohm’s fixed electrode geometry, variation of readings on a given sample is reduced from 10-30 Ω with STX2electrodes (depending on the experience of the user) to 1-2 Ω. Compared with other resistance measurement methods, Endohm with EVOM2™ offers a much more convenient and economic solution to “leaky tissue” measurement. Because of the uniform density of the AC square wave current from EVOM2™, errors owing to electrode polarization or membrane capacitance are largely eliminated. Endohm together with EVOM2™ offers the most accurate and economical endothelial ohmmeter now available. To date, cups from Costar, Millipore, ICN Biomedicals, and Falcon have been tested. Endohm chambers may be sterilized with EtO, alcohol or a bactericide; not autoclavable.

 

 

 

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Non-destructively test for epithelial monolayer confluence in 2D cell cultures