Szukaj

ibidi Gas incubation system

ibidi Gas incubation system

Manufacturer: 

A gas mixer that upgrades the ibidi Heating System to a complete stage top incubator for all live cell imaging applications with CO2, O2, and humidity control.

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ibidi Gas incubation system

  • Ideal for live cell imaging applications - full incubator conditions on the microscope
  • Stable gas incubation without evaporation (patent pending technology)
  • Suitable for various experimental conditions (e.g., pH or hypoxia)
  • Fully compatible with the ibidi Heating Systems  and stage top incubators of other suppliers
ibidi Gas Mixer Humidifying
column
Optional:
Air pressure gGenerator

Applications

  • Live cell imaging that requires incubator conditions on the microscope, for example:
    - Chemotaxis
    - Angiogenesis
    - Cell migration, proliferation, wound healing
    - Cell culture under flow
  • Imaging applications with extra focus stability, for example:
    - TIRF  
    - Confocal microscopy 
  • Anaerobic conditions or oxidative stress

Technical features

  • Precise and reliable gas incubation for O2 and CO2
  • Active and fast humidification - no evaporation
  • Uses pressurized air to create the gas flow – no vibrations
  • Optional air pressure generator available (for when pressurized air is not obtainable)

ibidi Gas mixer for CO2 and O2

  • Precise and reliable gas incubation
  • Active and fast humidification without evaporation (patent pending technology)
  • Suitable for various experimental conditions (e.g., pH or hypoxia)
  • Fully compatible with the ibidi Heating systems
  • Uses pressurized air to create the gas flow – no vibrations
  • Optional air pressure generator available (for when pressurized air is not obtainable)

Humidifying column

  • Part of the ibidi Gas incubation system
  • Humidifies the outgoing CO2 / O2 / air mix to minimize sample evaporation
  • Active humidity control inside the attached ibidi Heating system
Location/Tissue Oxygen Partial Pressure p(O2) Corresponding O2 in
Cell Culture Gas Mix (%)
Inspired air 21 kPa (=160 mm Hg) 21%
Alveolus 14 kPa (=104 mm Hg) 14%
Arterial blood 14 kPa (=104 mm Hg) 14%
Veneous blood 5 kPa (=40 mm Hg) 5%
Interstitial fluid 5 kPa (=40 mm Hg) 5%
Healthy tissue 2-3 kPa (=15- 23 mm Hg) 2-3%

Tumor without blood vessels

Outside
Center

 

1 kPa (=7.5 mm Hg)
0 kPa (=0 mm Hg)

 

1%
0%

Normoxia conditions 2 - 11 kPa (=15 - 82 mm Hg) 2-11%
Hypoxia conditions 0 - 1 kPa (=0 - 7.5 mm Hg) 0-1%
Cell culture incubator (5% CO2) 20 kPa (=150 mm Hg) 20%

Overview of the ibidi Heating & incubation system, CO2 and O2 control

Gas supply (input) - specification

 
Pressurized air 1 bar optimum / 14.5 psi
(0.8-1.2 bar / 11.6 – 17.4 psi)
CO2 1 bar optimum / 14.5 psi
(0.8-1.2 bar / 11.6 – 17.4 psi)
N2 1 bar optimum / 14.5 psi
(0.8-1.2 bar / 11.6 – 17.4 psi)
Tubing for all
gasses (Supplied with the system)
PUR/PUN tubing
4 mm inner diameter
6 mm outer diameter
1 mm wall thickness

ibidi Gas mixer - specification

 

 

 

CO2 control  
CO2 control range 0.1% - 20%
Accuracy 0.1% - 0.5% (absolute)
O2 control  
O2 control range 0% - 21%
Accuracy 0.5 % (absolute)
Gas flow  
Control  range 5 - 20 l/h

Humidifying column - specification

Humidity control  
Control range 20% - 99% (Rel. humidity)
Accuracy 1% (absolute)
Water refill Every 7 days

Ordering information

Cat. no. Description Pcs./box
11920 ibidi Gas incubation system for CO2: ibidi CO2 Gas mixer, Humidifying column 1
11922 ibidi Gas incubation system for CO2 and O2: ibidi CO2 and O2 Gas mixer, Humidifying column 1
11929 Air pressure generator, 1 bar, ready to use with the ibidi Gas incubation system 1